LOCUS       sequence-152823-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway cloning compatible binary vector for C-terminal fusion with 
            TAP (CaMV35S promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata 
            R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, 
            Ishiguro S
  TITLE     Improved Gateway binary vectors: high-performance vectors for 
            creation of fusion constructs in transgenic analysis of plants.
  JOURNAL   Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
  PUBMED    17690442
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci 
            Biotechnol Biochem."; date: "2007-08"; volume: "71(8)"; pages: 
            "2095-100. Epub 2007 Aug 7"
COMMENT     Sequence Label: pGWB429
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             244..321
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             349..369
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             409..582
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             583..756
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgataac
      241 agcaagagaa gatggaaaaa gaatttcata gccgtctcag cagccaaccg ctttaagaaa
      301 atctcatcct ccggggcact tgattatgat attccaacta ctgctagcga gaatttgtat
      361 tttcagggtg agctcaaaac cgcggctctt gcgcaacacg atgaagccgt ggacaacaaa
      421 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
      481 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
      541 ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac
      601 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa
      661 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca
      721 gaagctaaaa agctaaatgg tgctcaggcg ccgaaagtag acgcgaattc cgcggggaag
      781 tcaacct
//
