LOCUS       sequence-152825-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multiple cloning site for insertion of gene of interest for 
            C-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-MCS
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..127
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(167..183)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    194..317
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(388..405)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(388..404)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(397..419)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      468..554
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      646..673
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(832..849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcggccgcg ttaacgctac catggagctc caaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataat
      121 aaagttgcac tagttctaga cccggggaat tcgatatcaa gcttatcgat accgtcgacc
      181 tcgagggatc cagccacttt gtacaagaaa gttgaacgag aaacgtaaaa tgatataaat
      241 atcaatatat taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa
      301 catatgcagt cactatgaac caactactta gatggtatta gtgacctgta gaattcgagc
      361 tctagagctg cagggcccga gcttaagact ggccgtcgtt ttacaacgtc gtgactggga
      421 aaacatccat gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa
      481 aggctcagtc ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      541 tgagtaggac aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggagggt
      601 ggcgggcagg acgcccgcca taaactgcca ggcatcaaac taagcagaag gccatcctga
      661 cggatggcct ttttgcgttt ctacaaactc ttcctggcta gcggtacgcg tattaattgc
      721 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      781 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      841 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      901 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
      961 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1021 ccctgacgag catc
//
