LOCUS       sequence-152827-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multiple cloning site for insertion of gene of interest for 
            C-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-MCS
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(35..158)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     168..184
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     169..185
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(225..320)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(357..376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(358..376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(381..397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(381..397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(499..518)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(577..596)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctacaggtca ctaataccat ctaagtagtt ggttcatagt gactgcatat gttgtgtttt
       61 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc
      121 attttacgtt tctcgttcaa ctttcttgta caaagtggct ggatccctcg aggtcgacgg
      181 tatcgataag cttgatatcg aattccccgg gtctagaact agtgcaactt tattatacaa
      241 agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      301 tcaaaataaa atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct
      361 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
      421 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      481 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
      541 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      601 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
      661 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
      721 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
      781 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
      841 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
      901 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
      961 aatcacgaat gaataacggt ttggttgatg cgagtg
//
