LOCUS       sequence-152832-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mCherry (red) fluorophore with multiple cloning site for use with 
            inducible N-terminal fusions..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-mCherry-NS-MCS
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..126
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(166..182)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     regulatory      898..907
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    909..933
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcggccgcgt taacgctacc atggagctcc aaataatgat tttattttga ctgatagtga
       61 cctgttcgtt gcaacaaatt gataagcaat gcttttttat aatgccaact ttgtataata
      121 aagttgcact agttctagac ccggggaatt cgatatcaag cttatcgata ccgtcgacct
      181 cgagggatcc gtgcttgtac agctcgtcca ttccgcctgt agagtgtctg ccctcggccc
      241 gctcgtactg ttccacgatg gtgtagtcct cgttgtggct ggtgatgtcc agcttgatgt
      301 tcacgttgta ggcgccaggc agctgcacgg gtttcttggc cttgtaggtg gttttcactt
      361 cggcgtcgta gtggccgcca tccttcagtt tcagccgctg cttgatctcg cccttcaggg
      421 cgccatcctc agggtacatt ctctcgctgc tggcctccca gcccatggtc tttttctgca
      481 tcacgggtcc gtcgctgggg aagttggtgc cccgcagctt cactttgtag atgaactcgc
      541 cgtcctgcag agagctatcc tgggtcacgg tcacgacgcc gccgtcctcg aagttcatca
      601 ctctctccca cttgaagccc tcggggaagc tcagcttcag gtagtcgggg atgtcggcgg
      661 ggtgcttcac gtaggccttg ctgccgtaca taaactgggg gctcagaata tcccaggcga
      721 agggcagggg tccgcccttg gtcactttca gcttggcggt ctgtgttccc tcgtagggtc
      781 tgccttcgcc ctcgccctca atctcgaact cgtggccgtt cacgctgcct tccatgtgca
      841 ccttgaaccg catgaactct ttgatgatgg ccatgttgtc ctcttcgccc ttggacacca
      901 tggtggcagc cactttgtac aagaaagttg aacgagaaac gtaaaatgat ataaatatca
      961 atatattaaa ttagattttg cataaaaaac agactac
//
