LOCUS       sequence-152843-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Cetin A, Callaway EM
  TITLE     Optical control of retrogradely infected neurons using 
            drug-regulated ""TLoop"" lentiviral vectors.
  JOURNAL   J Neurophysiol. 2014 May;111(10):2150-9. doi: 10.1152/jn.00495.2013.
            Epub 2014 Feb 26.
  PUBMED    24572099
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/jn.00495.2013"; journalName: "J Neurophysiol"; date: 
            "2014-05"; volume: "111"; issue: "10"; pages: "2150-9"
COMMENT     Sequence Label: pEIAV-TLoop-Chr2-YFP
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..78
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             97..714
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
ORIGIN
        1 ctcggcatgg acgagctgta cgcggagggc cgcggcagcc tgctgacctg cggcgacgtg
       61 gaggagaacc ccggccccgc ccccggcagc ggtacctcca gactggacaa gagcaaagtc
      121 atcaactctg ccttggagct cctgaatgaa gttggcattg agggcttgac caccaggaag
      181 ctggcccaga agctgggtgt ggagcagcct accctgtact ggcatgtgaa gaacaagagg
      241 gctctgcttg atgccctggc cattgagatg ttggacaggc accacaccca cttctgccct
      301 ctggaagggg agtcctggca ggacttcctg aggaacaatg ccaagagctt cagatgtgcc
      361 ttgctctccc accgggatgg tgccaaagtt cacttgggca ccaggcctac agagaagcag
      421 tatgagaccc tggagaacca gctggcattc ctgtgccaac aaggcttctc cctggaaaat
      481 gccttgtatg ccctctctgc tgtgggccac ttcaccttgg gctgtgtgct ggaggaccag
      541 gagcaccaag ttgccaagga ggagagggag acccccacca ctgactccat gccaccactg
      601 ctgcggcaag ctattgagtt gtttgaccac caaggggctg agcctgcatt cctttttggc
      661 ctggaactga tcatctgtgg cctggaaaag cagctgaaat gtgagtctgg ctctcacgtg
      721 cccaaaaaga gaaagcacgt gcctgctgat gccttggatg attttgacct ggacatgctg
      781 cctgctgatg ccctggatga ctttgatttg gacatgctcc ctgctgatgc acttgatgat
      841 tttgacctgg atatgctgtg atccggagtc tacatttgaa tgtcaataaa acaaaatggt
      901 ggggcaatca tctacatttc atgg
//
