LOCUS       sequence-152846-         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent knockdown of Scn9a (Nav1.7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Branco T, Tozer A, Magnus CJ, Sugino K, Tanaka S, Lee AK, Wood JN, 
            Sternson SM
  TITLE     Near-Perfect Synaptic Integration by Nav1.7 in Hypothalamic Neurons 
            Regulates Body Weight.
  JOURNAL   Cell. 2016 Jun 16;165(7):1749-61. doi: 10.1016/j.cell.2016.05.019.
  PUBMED    27315482
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-16- 
            16"; volume: "165"; issue: "7"; pages: "1749-61"
COMMENT     Sequence Label: AAV-FLEX-EGFP-mir30(Scn9a)
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..72)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(117..150)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(117..150)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             161..880
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
ORIGIN
        1 aaattttgta atccagaggt tgattatcga taagcttgat aacttcgtat agtacacatt
       61 atacgaagtt atggctaagt ttgtgccatg ccattgatgc tacacagcct gaatggataa
      121 cttcgtatag gataccttat acgaagttat gctagccacc atggtgagca agcagatcct
      181 gaagaacacc ggcctgcagg agatcatgag cttcaaggtg aacctggagg gcgtggtgaa
      241 caaccacgtg ttcaccatgg agggctgcgg caagggcaac atcctgttcg gcaaccagct
      301 ggtgcagatc cgcgtgacca agggcgcccc cctgcccttc gccttcgaca tcctgagccc
      361 cgccttccag tacggcaacc gcaccttcac caagtacccc gaggacatca gcgacttctt
      421 catccagagc ttccccgccg gcttcgtgta cgagcgcacc ctgcgctacg aggacggcgg
      481 cctggtggag atccgcagcg acatcaacct gatcgaggag atgttcgtgt accgcgtgga
      541 gtacaagggc cgcaacttcc ccaacgacgg ccccgtgatg aagaagacca tcaccggcct
      601 gcagcccagc ttcgaggtgg tgtacatgaa cgacggcgtg ctggtgggcc aggtgatcct
      661 ggtgtaccgc ctgaacagcg gcaagttcta cagctgccac atgcgcaccc tgatgaagag
      721 caagggcgtg gtgaaggact tccccgagta ccacttcatc cagcaccgcc tggagaagac
      781 ctacgtggag gacggcggct tcgtggagca gcacgagacc gccatcgccc agctgaccag
      841 cctgggcaag cccctgggca gcctgcacga gtgggtgtaa caattgcagg tgagtggata
      901 tcaagccttg ttaagtgctc gcttcggca
//
