LOCUS       sequence-152850-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO construct expressing UBC for generation of GMAP-compatible 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: TOPO UBC
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     240..262
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     254..271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     255..271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     278..297
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        278..296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(581..600)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 acgtgcactg gccaggggga tcaccatccg tcgccccggc gtgtcaataa tatcactctg
       61 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgccg
      121 tacgtatagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      181 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      241 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
      301 attgggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc tgcagaattc
      361 cagcacactg gcggccgtta ctagtggatc ccgcttaagc tgtagcgcta atgtcgggtc
      421 ctagcgcgtt agcttcgtct aacaaaaaag ccaaaaacgg ccagaattta gcggacaatt
      481 tactagtcta acactgaaaa ttacatattg acccaaatga ttacatttca aaaggtgcct
      541 aaaaaacttc acaaaacaca ctcgccaacc ccgagcgcat agttcaaaac cggagcttca
      601 gctacttaag aagataggta cataaaaccg accaaagaaa ctgacgcctc acttatccct
      661 cccctcacca gaggtccggc gcctgtcgat tcaggagagc ctaccctagg cccgaaccct
      721 gcgtcctgcg acggagaaaa gcctaccgca cacctaccgg caggtggccc caccctgcat
      781 tataagccaa cagaacgggt gacgtcacga cacgacgagg gcgcgcgctc ccaaaggtac
      841 gggtgcactg cccaacggca ccgccataac tgccgccccc gcaacagacg acaaaccgag
      901 ttctccagtc agtgacaaac ttcacgtcag ggtccccaga tggtgcccca gcccatctca
      961 cccgaataag agctttcccg cattagcgaa ggcctcaaga ccttgggttc ttgccgccca
     1021 ccatgccccc caccttgttt
//
