LOCUS       sequence-152851-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DULIP Prey Destination Vector (Gateway, N-terminal).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Trepte P, Buntru A, Klockmeier K, Willmore L, Arumughan A, Secker C,
            Zenkner M, Brusendorf L, Rau K, Redel A, Wanker EE
  TITLE     DULIP: A Dual Luminescence-Based Co-Immunoprecipitation Assay for 
            Interactome Mapping in Mammalian Cells.
  JOURNAL   J Mol Biol. 2015 Oct 23;427(21):3375-88. doi: 
            10.1016/j.jmb.2015.08.003. Epub 2015 Aug 8.
  PUBMED    26264872
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2015.08.003"; journalName: "J Mol Biol"; date: 
            "2015-10-23- 23"; volume: "427"; issue: "21"; pages: "3375-88"
COMMENT     Sequence Label: pFL-V5-GW
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             126..167
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    180..304
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(329..334,335..352,353..359)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(479..498)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag
       61 agatcctcat aaaggccaag aagggcggaa agatcgccgt ggacgcgaat tccgcggggc
      121 tcgacggtaa gcctatccct aaccctctcc tcggtctcga ttctacgctc gaggatatca
      181 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      241 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
      301 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      361 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      421 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      481 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      541 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      601 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      661 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      721 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      781 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
      841 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
      901 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
      961 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggca
//
