LOCUS       sequence-152860-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of ISG15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Gonzalez-Sanz R, Mata M, Bermejo-Martin J, Alvarez A, Cortijo J, 
            Melero JA, Martinez I
  TITLE     ISG15 Is Upregulated in Respiratory Syncytial Virus Infection and 
            Reduces Virus Growth through Protein ISGylation.
  JOURNAL   J Virol. 2016 Jan 13;90(7):3428-38. doi: 10.1128/JVI.02695-15.
  PUBMED    26763998
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2016-01-13- 13"; volume: "90"; issue:
            "7"; pages: "3428-38"
COMMENT     Sequence Label: pCMV6-Neo-ISG15
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             146..940
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(200..219)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     810..829
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
       61 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
      121 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
      181 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
      241 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
      301 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
      361 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
      421 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
      481 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
      541 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
      601 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
      661 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
      721 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
      781 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
      841 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
      901 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
      961 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattcc
//
