LOCUS       sequence-152863-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression of nonconjugative ISG15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Gonzalez-Sanz R, Mata M, Bermejo-Martin J, Alvarez A, Cortijo J, 
            Melero JA, Martinez I
  TITLE     ISG15 Is Upregulated in Respiratory Syncytial Virus Infection and 
            Reduces Virus Growth through Protein ISGylation.
  JOURNAL   J Virol. 2016 Jan 13;90(7):3428-38. doi: 10.1128/JVI.02695-15.
  PUBMED    26763998
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2016-01-13- 13"; volume: "90"; issue:
            "7"; pages: "3428-38"
COMMENT     Sequence Label: pCMV6-Neo-ISG15-LRAA
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(731..747)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(731..747)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac tcactatagg
       61 gcggccgcgg tgctgcctgc cgaagccggc ggctgagagg cagcgaactc atctttgcca
      121 gtacaggagc tcgtgccgtg gcccacagcc cacagcccac agccatgggc tgggacctga
      181 cggtgaagat gctggcgggc aacgaattcc aggtgtccct gagcagctcc atgtcggtgt
      241 cagagctgaa ggcgcagatc acccagaaga tcggcgtgca cgccttccag cagcgtctgg
      301 ctgtccaccc gagcggtgtg gcgctgcagg acagggtccc ccttgccagc cagggcctgg
      361 gccccggcag cacggtcctg ctggtggtgg acaaatgcga cgaacctctg agcatcctgg
      421 tgaggaataa caagggccgc agcagcacct acgaggtgcg gctgacgcag accgtggccc
      481 acctgaagca gcaagtgagc gggctggagg gtgtgcagga cgacctgttc tggctgacct
      541 tcgaggggaa gcccctggag gaccagctcc cgctggggga gtacggcctc aagcccctga
      601 gcaccgtgtt catgaatctg cgcctgcggg cagccggcac agagcctggc gggcggagct
      661 aagggcctcc accagcatcc gagcaggatc aagggccgga aataaaggct gttgtaaaga
      721 ggcggccgcg gtcatagctg tttcctgaac agatcccggg tggcatccct gtgacccctc
      781 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
      841 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtgga
//
