LOCUS       sequence-152865-         297 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent knockdown of Scn9a (Nav1.7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Branco T, Tozer A, Magnus CJ, Sugino K, Tanaka S, Lee AK, Wood JN, 
            Sternson SM
  TITLE     Near-Perfect Synaptic Integration by Nav1.7 in Hypothalamic Neurons 
            Regulates Body Weight.
  JOURNAL   Cell. 2016 Jun 16;165(7):1749-61. doi: 10.1016/j.cell.2016.05.019.
  PUBMED    27315482
REFERENCE   2  (bases 1 to 297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05"; journalName: "Cell"; date: "2016-06-16- 
            16"; volume: "165"; issue: "7"; pages: "1749-61"
COMMENT     Sequence Label: AAV-FLEX-EGFP-mir30(Scn9a)
FEATURES             Location/Qualifiers
     source          1..297
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(6..39)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(6..39)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(58..91)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           complement(162..288)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 cctccataac ttcgtatagg ataccttata cgaagttatc tcagtttaaa ctaagccata
       61 acttcgtata gtacacatta tacgaagtta ttgtttaccg gtacctggag gaaaaaaaaa
      121 aggggagagt caggggtcgc gaattagtct tccaattgaa aaaagtgatt taatttatac
      181 cattttaatt cagctttgta aaaatgtatc aaagagatag caaggtattc agttttagta
      241 aacaagataa ttgctcctaa agtagcccct tgaattccga ggcagtaggc atgggtg
//
