LOCUS       sequence-152881-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-SH3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-dCas9-SH3
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..104)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaattcgctt attaggatcc ttatccggag cctctagact tatcgtcatc gtctttgtaa
       61 tcaatatcat gatccttgta gtctccgtcg tggtccttat agtctccgga atacttctcc
      121 acgtaaggga caggaatcat ccccctcttt ccttcgctgt cctctgcatt ccaccactgc
      181 tcttcaggct tatcccggat tctcaggatg tctcctttct taaagggaag atcttcttca
      241 tcattcccat taaagtcaaa gagggcccgc acatactctg cagaacctcc acctccagaa
      301 cctcctccac ccaccttcct cttcttcttg gggtcagccc tgctgtctcc accgagctga
      361 gagaggtcga ttcttgtttc atagagcccc gtaattgact gatgaatcag tgtggcgtcc
      421 aggacctcct ttgtagaggt gtaccgcttt ctgtctatgg tggtgtcgaa gtacttgaag
      481 gctgcaggcg cgcccaagtt ggtcagagta aacaagtgga taatgttttc tgcctgctcc
      541 ctgatgggct tatccctgtg cttattgtaa gcagaaagca ccttatcgag gttagcgtcg
      601 gcgaggatca ctcttttgga gaattcgctt atttgctcga tgatctcatc aaggtagtgt
      661 ttgtgttgtt ccacgaacag ctgcttctgc tcattatctt cgggagaccc tttgagcttt
      721 tcatagtggc tggccagata caagaaatta acgtatttag agggcagtgc cagctcgtta
      781 cctttctgca gctcgcc
//
