LOCUS       sequence-152883-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Saporin L3 in pichia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Yuan H, Du Q, Sturm MB, Schramm VL
  TITLE     Soapwort Saporin L3 Expression in Yeast, Mutagenesis, and RNA 
            Substrate Specificity.
  JOURNAL   Biochemistry. 2015 Jul 28;54(29):4565-74. doi: 
            10.1021/acs.biochem.5b00405. Epub 2015 Jul 14.
  PUBMED    26091305
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00405"; journalName: "Biochemistry"; date: 
            "2015-07-28- 28"; volume: "54"; issue: "29"; pages: "4565-74"
COMMENT     Sequence Label: pPICZalphaB-SapL3
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..66)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(82..111)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gtgcccaact tgaactgagg aacagtcatg tctaaggcta caaactcaat gatgatgatg
       61 atgatggtcg acggcgctat tcagatcctc ttctgagatg agtttttgtt ctagaaagct
      121 ggcggccgca ttgttgtcta cgttcagaaa gatccctatt tccacttccg ccacagtagt
      181 caccctccac tcgacgccag ggacagcata acttgtcaaa acaagcggag tttggaacac
      241 tccattgttt gcagttttgg cagctcttga aagcagaccc caattatttt ccaatataat
      301 cacacgatca ggaacgggtt caacctcctt tgctgtatca aaattgttaa gtaccaaatt
      361 ctctatgtac ttaaatcgcg ctgcttcacc caccatttga acaacgatca acaaaaacag
      421 tgcttcatct ttttcaacac gcgcaacccc gttcactttt gtcatggact cagcaagttt
      481 cttgatcccc aagcccgctt gctgtcttgt taccccggct gcgttttgta tttgcggata
      541 tgattcacca taccctaatt cttgctgatt tgagacacca gttgcctctg ggaaaaggtt
      601 gtttaactga ttcgtagtaa tttggaagcc cttaaagtaa tatgcccgaa attgtttgtt
      661 gttattctta gcaagatacg cggccacata caagttacta cgctgtacgg caagtgagac
      721 agttccagta gaagctttga gattaactct aaggaatttc ggggggccca caggtctttt
      781 tatcacgggt agattggtac caccgtaatg gaggtttgga tcctttatat cgtcacggag
      841 ctgtt
//
