LOCUS       sequence-152884-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..121)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(329..349)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt aggatcctta tccggagcct ctagacttat
       61 cgtcatcgtc tttgtaatca atatcatgat ccttgtagtc tccgtcgtgg tccttatagt
      121 ctccggaata cttctccacg taagggacag gaatcatccc cctctttcct tcgctgtcct
      181 ctgcattcca ccactgctct tcaggcttat cccggattct caggatgtct cctttcttaa
      241 agggaagatc ttcttcatca ttcccattaa agtcaaagag ggcccgcaca tactctgcag
      301 aacctccacc tccagaacct cctccaccca ccttcctctt cttcttgggg tcagccctgc
      361 tgtctccacc gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat
      421 gaatcagtgt ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg
      481 tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa
      541 tgttttctgc ctgctccctg atgggcttat ccctgtgctt attgtaagca gaaagcacct
      601 tatcgaggtt agcgtcggcg aggatcactc ttttggagaa ttcgcttatt tgctcgatga
      661 tctcatcaag gtagtgtttg tgttgttcca cgaacagctg cttctgctca ttatcttcgg
      721 gagacccttt gagcttttca tagtggctgg ccagatacaa gaaattaacg tatttagagg
//
