LOCUS       sequence-152889-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-dCas9-PmCDA1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..683)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(690..755)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ataaaaatca taaatcataa gaaattcgct tattaggatc cttatccgga gcctctagaa
       61 acagcaggac tcttagtggt gtggagtatt tttacctgaa tcataatgga caactcgctc
      121 cgtcgttttt cagctcgctt caaagtcttc tcaagccatc tattctcatt caattgattg
      181 tgcgacgatt ggatgaatat tttcctgcaa cattggtagt gttcacttac cattacattc
      241 aacccaaccc cgttatctct gaggttccac agcccaattt gattcctcgc atttttctcg
      301 taatagagtt tgcaagccca gattttcaaa gtgtggccgt tcccccgcag ctcctggtta
      361 taccattcta agatcttttc agcgcaatct gcacaaggac tccaggatga gtaccaattt
      421 atcgtgaatt gtccggggtt gtcgcgcagg tattcttcga cttttctaat gctaaagatt
      481 tcggcgtgaa tgccacgttc tgtcccgctc tgtggtttat tcacagcata gccccaaaaa
      541 cacgctctac gttcaccccg tcgttttaat tcaaagagaa cgtagcatct atgcgacacg
      601 gattttttgt tgttgaaaaa ctgtttctta aacgtgtaga tgtccaactt ctcatggatt
      661 ctcacgtact cagcgtcggt catcctagac ttatcgtcat cgtctttgta atcaatatca
      721 tgatccttgt agtctccgtc gtggtcctta tagtctccgg aatacttctc cacgtaagg
//
