LOCUS       sequence-152895-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(H840A) in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS415_pGal-nCas9(H840A)
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(480..499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     599..621
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(659..677)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt
       61 acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac atacatttat
      121 caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat actaatggct
      181 caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga ggagggcacc
      241 acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt tatatattgg
      301 aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat
      361 ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc
      421 aagaatttta ctctgtcaga aacggcctta acgacgtagt cgatatggtg cactctcagt
      481 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      541 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      601 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
      661 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga
      721 tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt
      781 tactctgtgt ttatttattt ttatgttttg tatttggatt ttagaa
//
