LOCUS       sequence-152898-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(D10A)-PmCDA1 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-nCas9(D10A)-PmCDA1
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..679)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(686..751)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aaatcataaa tcataagaaa ttcgcttatt aggatcctta tccggagcct ctagaaacag
       61 caggactctt agtggtgtgg agtattttta cctgaatcat aatggacaac tcgctccgtc
      121 gtttttcagc tcgcttcaaa gtcttctcaa gccatctatt ctcattcaat tgattgtgcg
      181 acgattggat gaatattttc ctgcaacatt ggtagtgttc acttaccatt acattcaacc
      241 caaccccgtt atctctgagg ttccacagcc caatttgatt cctcgcattt ttctcgtaat
      301 agagtttgca agcccagatt ttcaaagtgt ggccgttccc ccgcagctcc tggttatacc
      361 attctaagat cttttcagcg caatctgcac aaggactcca ggatgagtac caatttatcg
      421 tgaattgtcc ggggttgtcg cgcaggtatt cttcgacttt tctaatgcta aagatttcgg
      481 cgtgaatgcc acgttctgtc ccgctctgtg gtttattcac agcatagccc caaaaacacg
      541 ctctacgttc accccgtcgt tttaattcaa agagaacgta gcatctatgc gacacggatt
      601 ttttgttgtt gaaaaactgt ttcttaaacg tgtagatgtc caacttctca tggattctca
      661 cgtactcagc gtcggtcatc ctagacttat cgtcatcgtc tttgtaatca atatcatgat
      721 ccttgtagtc tccgtcgtgg tccttatagt ctccggaata cttctccacg taagggacag
      781 gaatcatcc
//
