LOCUS       sequence-152902-         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(D10A) in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS415_pGal-nCas9(D10A)
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(102..113)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cagattatcg aacttccgtt gtgtgatcag tttggcgttc agcagctgcc gccaataatt
       61 tttcattttc ttgacaactt cttctgaggg gacgttatca ctcttccctc tatttttatc
      121 ggatcttgtc aacactttat tatcaataga atcatctttg agaaaagact ggggcacgat
      181 atgatccacg tcgtagtcgg agagccgatt gatgtccagt tcctgatcca cgtacatgtc
      241 cctgccgttc tgcaggtagt acaggtagag cttctcattc tgaagctggg tgttttcaac
      301 tgggtgttcc ttaaggattt gggaccccag ttcttttata ccctcttcaa tcctcttcat
      361 cctttcccta ctgttcttct gtcccttctg ggtagtttgg ttctctcggg ccatctcgat
      421 aacgatattc tcgggcttat gccttcccat tactttgacg agttcatcca cgaccttaac
      481 ggtctgcagt attccctttt tgatagctgg gctacctgca agattagcga tgtgctcgtg
      541 aagactgtcc ccctggccag aaacttgtgc tttctggatg tcctccttaa aggtgagaga
      601 gtcatcatgg atcaactgca tgaagttccg gttggcaaat ccatcggact taagaaaatc
      661 caggattgtc tttccactct gcttgtctcg gatcccattg atcagttttc ttgacagccg
      721 cccccatcct gtatatcggc gcctcttgag ctgtttcatg actttgtcgt cga
//
