LOCUS       sequence-152903-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(D10A) in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS415_pGal-nCas9(D10A)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        80..756
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    308..425
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     720..743
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 agggaacaaa agctggtacc gggcccgaat tctctccttc tcttaggtgg cagagcaggt
       61 ggagggtcga ccatactagt ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa
      121 gtttaataat catattacat ggcattacca ccatatacat atccatatac atatccatat
      181 acatatccat atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta
      241 gcctaaaaaa accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat
      301 tgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc
      361 gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg
      421 ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc
      481 agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata
      541 atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg
      601 atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac
      661 attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta
      721 atatacctct atactttaac gtcaaggaga aaaaacccat ggacaagaag tactccattg
      781 ggctcgctat cggcacaaac agcgtcggtt gggccgtcat tacggacgag tacaag
//
