LOCUS       sequence-152904-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(D10A) in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS415_pGal-nCas9(D10A)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(490..509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     609..631
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(669..687)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat aatagaaacg
       61 acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata cgcaatctac
      121 atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa gcaaattgat
      181 actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata ttgacaagga
      241 ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg attcctaatt
      301 tatatattgg aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg
      361 acctgaccat ttgatggagt ttaagtcaat accttcttga accatttccc ataatggtga
      421 aagttccctc aagaatttta ctctgtcaga aacggcctta acgacgtagt cgatatggtg
      481 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      541 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      601 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      661 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      721 ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt atcttttaat gatggaataa
      781 tttgggaatt tactctgtgt ttatttattt ttatgttttg tatttggatt
//
