LOCUS       sequence-152907-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(99..110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 attatcgaac ttccgttgtg tgatcagttt ggcgttcagc agctgccgcc aataattttt
       61 cattttcttg acaacttctt ctgaggggac gttatcactc ttccctctat ttttatcgga
      121 tcttgtcaac actttattat caatagaatc atctttgaga aaagactggg gcacgatatg
      181 atccacgtcg tagtcggaga gccgattgat gtccagttcc tgatccacgt acatgtccct
      241 gccgttctgc aggtagtaca ggtagagctt ctcattctga agctgggtgt tttcaactgg
      301 gtgttcctta aggatttggg accccagttc ttttataccc tcttcaatcc tcttcatcct
      361 ttccctactg ttcttctgtc ccttctgggt agtttggttc tctcgggcca tctcgataac
      421 gatattctcg ggcttatgcc ttcccattac tttgacgagt tcatccacga ccttaacggt
      481 ctgcagtatt ccctttttga tagctgggct acctgcaaga ttagcgatgt gctcgtgaag
      541 actgtccccc tggccagaaa cttgtgcttt ctggatgtcc tccttaaagg tgagagagtc
      601 atcatggatc aactgcatga agttccggtt ggcaaatcca tcggacttaa gaaaatccag
      661 gattgtcttt ccactctgct tgtctcggat cccattgatc agttttcttg acagccgccc
      721 ccatcctgta tatcggcgcc tcttgagctg tttcatgact t
//
