LOCUS       sequence-152908-         784 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..784
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..78)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(85..105)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(739..762)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccatcttgt tcaatcattc tagatggacc agggttttct tccacatccc cacaagtcag
       61 aaggcttccc ctgccttcgg taccgacttt tcttttcttc ttgggaccaa cggctggaga
      121 cttagtggta tgcagaatct tgacctggat catgatgctc agctcggatc tccacttctc
      181 cgcacgtttc agtgttttct ccaaccagcg gttttcattg agctggttat gagagctttg
      241 gatgaaaatc ttccgacaac actgatagtg ctcggaaacc atcacgttca ggcctacacc
      301 attgtccctc agattccaga gtcctatctg gttccgggca ttcttctcgt agtacagctt
      361 gcaggcccag attttcaatg tgtgcccgtt tccccgaagt tcctgattat accattccag
      421 aattttctcg gcacaatcag cgcaaggact ccagctggaa taccagttga tcgtaaactg
      481 cccaggatta tcccgaagat actcttccac ctttcggata ctgaatatct cagcgtgtat
      541 tcctctctcg gtgccacttt ggggcttgtt gacggcatag ccccaaaagc aagcccttct
      601 ttcgcccctt cgcttcaact caaacagcac gtagcagcgg tgtgacacag atttcttgtt
      661 gttgaagaac tgcttcttga aggtgtaaat atccagtttc tcatgaatgc gcacgtactc
      721 ggcgtctgtc atccctgatt tatcgtcgtc gtctttgtag tcgatgtcgt ggtctttgta
      781 atcc
//
