LOCUS       sequence-152910-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses super ecliptic A227D in mammalian cell membranes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kang BE, Baker BJ
  TITLE     Pado, a fluorescent protein with proton channel activity can 
            optically monitor membrane potential, intracellular pH, and map gap 
            junctions.
  JOURNAL   Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865.
  PUBMED    27040905
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-4"; volume: "6:23865. doi"; pages: " 
            10.1038/srep23865"
COMMENT     Sequence Label: Farnesylated SE pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..33
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14..32
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             121..834
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(151..179)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     774..794
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcttatcga aattaatacg actcactata gggagaccca agctggctag cgtttaaact
       61 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcgc
      121 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      181 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      241 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      301 gtcactactt taacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg
      361 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      421 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      481 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      541 ttggaataca actataacga tcaccaggtg tacatcatgg cagacaaaca aaagaatgga
      601 atcaaagcta acttcaaaat tagacacaac attgaagatg gaggcgttca actagcagac
      661 cattatcaac aaaatactcc aattggcgat gggcccgtcc ttttaccaga caaccattac
      721 ctgtttacaa cttctactct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      781 cttgagtttg taacagctga tgggattaca catggcatgg atgaactata caaatccgga
      841 ctcagatcta agctgaaccc tcc
//
