LOCUS       sequence-152912-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..115)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtcagattat cgaacttccg ttgtgtgatc agtttggcgt tcagcagctg ccgccaataa
       61 tttttcattt tcttgacaac ttcttctgag gggacgttat cactcttccc tctattttta
      121 tcggatcttg tcaacacttt attatcaata gaatcatctt tgagaaaaga ctggggcacg
      181 atatgatcca cgtcgtagtc ggagagccga ttgatgtcca gttcctgatc cacgtacatg
      241 tccctgccgt tctgcaggta gtacaggtag agcttctcat tctgaagctg ggtgttttca
      301 actgggtgtt ccttaaggat ttgggacccc agttctttta taccctcttc aatcctcttc
      361 atcctttccc tactgttctt ctgtcccttc tgggtagttt ggttctctcg ggccatctcg
      421 ataacgatat tctcgggctt atgccttccc attactttga cgagttcatc cacgacctta
      481 acggtctgca gtattccctt tttgatagct gggctacctg caagattagc gatgtgctcg
      541 tgaagactgt ccccctggcc agaaacttgt gctttctgga tgtcctcctt aaaggtgaga
      601 gagtcatcat ggatcaactg catgaagttc cggttggcaa atccatcgga cttaagaaaa
      661 tccaggattg tctttccact ctgcttgtct cggatcccat tgatcagttt tcttgacagc
      721 cgcccccatc ctgtatatcg gcgcctcttg agctgtttca tgactttgtc gtcgaagaga
      781 tgagcgtaag ttttcaagcg ttcttcaatc atctcc
//
