LOCUS       sequence-152919-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..82)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(89..109)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(113..736)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(743..766)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcaatccatc ttgttcaatc attctagatg gaccagggtt ttcttccaca tccccacaag
       61 tcagaaggct tcccctgcct tcggtaccga cttttctttt cttcttggga ccaacggctg
      121 gagacttagt ggtatgcaga atcttgacct ggatcatgat gctcagctcg gatctccgct
      181 tctccgcacg tttcagtgtt ttctccaacc agcggttttc attgagctgg ttatgagagc
      241 tttggatgaa aatcttccga caacactgat agtgctcgga aaccatcacg ttcaggccta
      301 caccattgtc cctcagattc cagagtccta tctggttccg ggcattcttc tcgtagtaca
      361 gcttgcaggc ccagattttc aatgtgtgcc cgtttccccg aagttcctga ttataccatt
      421 ccagaatttt ctcggcacaa tcagcgcaag gactccagct ggaataccag ttgatcgtaa
      481 actgcccagg attatcccga agatactctt ccacctttcg gatactgaat atctcagcgt
      541 gtattcctct ctcggtgcca ctttggggct tgttgacggc atagccccaa aagcaagccc
      601 ttctttcgcc ccttcgcttc aactcaaaca gcacgtagca gcggtgtgac acagatttct
      661 tgttgttgaa gaactgcttc ttgaaggtgt aaatatccag tttctcatga atgcgcacgt
      721 actcggcgtc tgtcatccct gatttatcgt cgtcgtcttt gtagtcgatg tcgtggtctt
      781 tgtaatcc
//
