LOCUS       sequence-152920-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(106..117)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tagtcagatt atcgaacttc cgttgtgtga tcagtttggc gttcagcagc tgccgccaat
       61 aatttttcat tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt
      121 tatcggatct tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca
      181 cgatagcatc cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca
      241 tgtccctgcc gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt
      301 caactgggtg ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct
      361 tcatcctttc cctactgttc ttctgtccct tctgggtagt ttggttctct cgggccatct
      421 cgataacgat attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct
      481 taacggtctg cagtattccc tttttgatag ctgggctacc tgcaagatta gcgatgtgct
      541 cgtgaagact gtccccctgg ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga
      601 gagagtcatc atggatcaac tgcatgaagt tccggttggc aaatccatcg gacttaagaa
      661 aatccaggat tgtctttcca ctctgcttgt ctcggatccc attgatcagt tttcttgaca
      721 gccgccccca tcctgtatat cggcgcctct tgagctgttt catgactttg tcgtcga
//
