LOCUS       sequence-152921-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with 
            GST (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Menade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S,
            Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young 
            JC, Gehring K
  TITLE     Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin 
            provide insight into pathological mutations.
  JOURNAL   J Biol Chem. 2018 Aug 17;293(33):12832-12842. doi: 
            10.1074/jbc.RA118.003939. Epub 2018 Jun 26.
  PUBMED    29945973
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.003939"; journalName: "J Biol Chem"; date: 
            "2018-08-17- 17"; volume: "293"; issue: "33"; pages: "12832-12842"
COMMENT     Sequence Label: pGEX6P1-sr1(SIRPT1)
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..32
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(878..900)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 aatcggatct ggaagttctg ttccaggggc ccctgggatc caaaggggga ggtcgatttg
       61 gtcagacaac gccaccactt gttgattttc tcaaggacat tttgagaaga tatccagaag
      121 gaggacagat tcttaaggaa ttaattcaga atgcagaaga tgctggggcg acagaagtta
      181 aatttttata tgatgaaact caatacggaa cagagactct ttggtcaaaa gatatggcgc
      241 catatcaggg gccagctctc tatgtgtaca acaacgcggt tttcacccca gaggactggc
      301 acggcattca agaaatagca agaagcagga aaaaggatga tcctctgaag gtcggaagat
      361 ttggaattgg gtttaattct gtctatcata taacagatgt tccttgtatc tttagtggtg
      421 accaaatcgg gatgctagat cctcatcaaa cactttttgg cccacatgaa tcaggccaat
      481 gttggaatct caaagatgac agcaaagaaa ttagtgaact ttcagaccag tttgcaccat
      541 ttgttggcat ttttggaagc accaaggaaa catttataaa cggcaatttt ccaggaacat
      601 ttttccgttt ccctcttcgc ctacaacctt cacaacttag tagtaacctc tacaataagc
      661 agaaggttct tgagttgttt gagtctttta gggcagatgc ggacacagtg ctgctctttc
      721 tgaaaagtgt gcaggatgtt tccttatatg tccgagaggc tgacggaaca gagaaactgg
      781 tgtttagagt gacttcgagt gagagtaagt agctcgagcg gccgcatcgt gactgactga
      841 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      901 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      961 ca
//
