LOCUS       sequence-152923-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-dCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(103..114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcagattatc gaacttccgt tgtgtgatca gtttggcgtt cagcagctgc cgccaataat
       61 ttttcatttt cttgacaact tcttctgagg ggacgttatc actcttccct ctatttttat
      121 cggatcttgt caacacttta ttatcaatag aatcatcttt gagaaaagac tggggcacga
      181 tagcatccac gtcgtagtcg gagagccgat tgatgtccag ttcctgatcc acgtacatgt
      241 ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt ctgaagctgg gtgttttcaa
      301 ctgggtgttc cttaaggatt tgggacccca gttcttttat accctcttca atcctcttca
      361 tcctttccct actgttcttc tgtcccttct gggtagtttg gttctctcgg gccatctcga
      421 taacgatatt ctcgggctta tgccttccca ttactttgac gagttcatcc acgaccttaa
      481 cggtctgcag tattcccttt ttgatagctg ggctacctgc aagattagcg atgtgctcgt
      541 gaagactgtc cccctggcca gaaacttgtg ctttctggat gtcctcctta aaggtgagag
      601 agtcatcatg gatcaactgc atgaagttcc ggttggcaaa tccatcggac ttaagaaaat
      661 ccaggattgt ctttccactc tgcttgtctc ggatcccatt gatcagtttt cttgacagcc
      721 gcccccatcc tgtatatcgg cgcctcttga gctgtttcat gactttgtcg tcgaagagat
      781 gagcgtaagt tttcaagcgt tcttcaatca tctccctatc ttcaa
//
