LOCUS       sequence-152924-         958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with 
            GST (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Menade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S,
            Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young 
            JC, Gehring K
  TITLE     Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin 
            provide insight into pathological mutations.
  JOURNAL   J Biol Chem. 2018 Aug 17;293(33):12832-12842. doi: 
            10.1074/jbc.RA118.003939. Epub 2018 Jun 26.
  PUBMED    29945973
REFERENCE   2  (bases 1 to 958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.003939"; journalName: "J Biol Chem"; date: 
            "2018-08-17- 17"; volume: "293"; issue: "33"; pages: "12832-12842"
COMMENT     Sequence Label: pGEX6P1-sr1(SIRPT1)
FEATURES             Location/Qualifiers
     source          1..958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(827..850)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(877..899)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 aacgcgcgag gcagatcgtc agtcagtcac gatgcggccg ctcgagctac ttactctcac
       61 tcgaagtcac tctaaacacc agtttctctg ttccgtcagc ctctcggaca tataaggaaa
      121 catcctgcac acttttcaga aagagcagca ctgtgtccgc atctgcccta aaagactcaa
      181 acaactcaag aaccttctgc ttattgtaga ggttactact aagttgtgaa ggttgtaggc
      241 gaagagggaa acggaaaaat gttcctggaa aattgccgtt tataaatgtt tccttggtgc
      301 ttccaaaaat gccaacaaat ggtgcaaact ggtctgaaag ttcactaatt tctttgctgt
      361 catctttgag attccaacat tggcctgatt catgtgggcc aaaaagtgtt tgatgaggat
      421 ctagcatccc gatttggtca ccactaaaga tacaaggaac atctgttata tgatagacag
      481 aattaaaccc aattccaaat cttccgacct tcagaggatc atcctttttc ctgcttcttg
      541 ctatttcttg aatgccgtgc cagtcctctg gggtgaaaac cgcgttgttg tacacataga
      601 gagctggccc ctgatatggc gccatatctt ttgaccaaag agtctctgtt ccgtattgag
      661 tttcatcata taaaaattta acttctgtcg ccccagcatc ttctgcattc tgaattaatt
      721 ccttaagaat ctgtcctcct tctggatatc ttctcaaaat gtccttgaga aaatcaacaa
      781 gtggtggcgt tgtctgacca aatcgacctc cccctttgga tcccaggggc ccctggaaca
      841 gaacttccag atccgatttt ggaggatggt cgccaccacc aaacgtggct tgccagccct
      901 gcaaaggcca tgctatatac ttgctggatt tcaagtactt atcaatttgt gggatagc
//
