LOCUS       sequence-152932-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sacsin HEPN domain (4441-4579) in fusion with GST 
            (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Kozlov G, Denisov AY, Girard M, Dicaire MJ, Hamlin J, McPherson PS, 
            Brais B, Gehring K
  TITLE     Structural basis of defects in the sacsin HEPN domain responsible 
            for autosomal recessive spastic ataxia of Charlevoix-Saguenay 
            (ARSACS).
  JOURNAL   J Biol Chem. 2011 Jun 10;286(23):20407-12. doi: 
            10.1074/jbc.M111.232884. Epub 2011 Apr 20.
  PUBMED    21507954
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.232884"; journalName: "J Biol Chem"; date: 
            "2011-06-10- 10"; volume: "286"; issue: "23"; pages: "20407-12"
COMMENT     Sequence Label: pGEX4T-HEPN
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(515..537)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(682..700)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        768..872
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggttccgcgt ggatccatgg ttggcaatcc agtggaagca cgcagatggc
       61 taagacaagc cagagcaaac ttctcagctg ccaggaatga ccttcataaa aatgccaatg
      121 agtgggtgtg ctttaaatgt tacctttcta ccaagttagc tttgattgca gctgactatg
      181 ctgtgagggg aaagtctgat aaagatgtaa aaccaactgc acttgctcag aaaatagagg
      241 aatatagtca gcaacttgaa ggactgacaa atgatgttca cacattggaa gcttatggtg
      301 tagacagttt aaaaacaaga taccctgatt tgcttccctt tcctcagatc ccaaatgaca
      361 ggttcacttc tgaggttgct atgagggtga tggaatgtac tgcctgtatc ataataaaac
      421 ttgaaaattt tatgcaacaa aaagtgtaac tcgagcggcc gcatcgtgac tgactgacga
      481 tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      541 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
      601 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
      661 ataattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      721 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      781 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      841 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      901 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      961 aagtaaaaga tgctgaagat cagttgggtg
//
