LOCUS       sequence-152933-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Sacsin HEPN domain (4441-4579) in fusion with GST 
            (bacterial).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Kozlov G, Denisov AY, Girard M, Dicaire MJ, Hamlin J, McPherson PS, 
            Brais B, Gehring K
  TITLE     Structural basis of defects in the sacsin HEPN domain responsible 
            for autosomal recessive spastic ataxia of Charlevoix-Saguenay 
            (ARSACS).
  JOURNAL   J Biol Chem. 2011 Jun 10;286(23):20407-12. doi: 
            10.1074/jbc.M111.232884. Epub 2011 Apr 20.
  PUBMED    21507954
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.232884"; journalName: "J Biol Chem"; date: 
            "2011-06-10- 10"; volume: "286"; issue: "23"; pages: "20407-12"
COMMENT     Sequence Label: pGEX4T-HEPN
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(401..418)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(445..467)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tttcaagttt tattatgata caggcagtac attccatcac cctcatagca acctcagaag
       61 tgaacctgtc atttgggatc tgaggaaagg gaagcaaatc agggtatctt gtttttaaac
      121 tgtctacacc ataagcttcc aatgtgtgaa catcatttgt cagtccttca agttgctgac
      181 tatattcctc tattttctga gcaagtgcag ttggttttac atctttatca gactttcccc
      241 tcacagcata gtcagctgca atcaaagcta acttggtaga aaggtaacat ttaaagcaca
      301 cccactcatt ggcattttta tgaaggtcat tcctggcagc tgagaagttt gctctggctt
      361 gtcttagcca tctgcgtgct tccactggat tgccaaccat ggatccacgc ggaaccagat
      421 ccgattttgg aggatggtcg ccaccaccaa acgtggcttg ccagccctgc aaaggccatg
      481 ctatatactt gctggatttc aagtacttat caatttgtgg gatagcttca atacgttttt
      541 taaaacaaac taattttggg aacgcatcca ggcacattgg gtccatgtat aaaacaacat
      601 caagagcgtc atacaacatg aagtcaggat gggttacatg atcaccattt aaatatgttt
      661 tatgacataa acgatcttcg aacattttca gcatttcagg tagcttgcta agaaaatcaa
      721 ctttgagagt ttcaaagtct ttactatatg caattctcga aacaccgtat ctaatatcca
      781 aaaccgctcc ttcaagcatt gaaatctctg cacgctc
//
