LOCUS       sequence-152941-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mutant AlkB protein (D135S) in E. coli BL21(DE3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Zheng G, Qin Y, Clark WC, Dai Q, Yi C, He C, Lambowitz AM, Pan T
  TITLE     Efficient and quantitative high-throughput tRNA sequencing.
  JOURNAL   Nat Methods. 2015 Sep;12(9):835-7. doi: 10.1038/nmeth.3478. Epub 
            2015 Jul 27.
  PUBMED    26214130
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3478"; journalName: "Nat Methods"; date: "2015-09"; 
            volume: "12"; issue: "9"; pages: "835-7"
COMMENT     Sequence Label: pET30a-AlkB-D135S
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             723..740
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(793..811)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgca agagccactg gcggctggag
      121 cggtaatttt acggcgtttt gcttttaacg ctgcggagca actaatccgc gatattaatg
      181 acgttgccag ccagtcgccg tttcgccaga tggtcacccc cgggggatat accatgtcgg
      241 tggcgatgac caactgtggg catctgggct ggacgaccca tcggcaaggt tatctctatt
      301 cgcccattga tccgcaaaca aataaaccgt ggcccgccat gccacagagt tttcataatt
      361 tatgtcaacg tgcggctacg gcggcgggct atccagattt ccagccagat gcttgtctta
      421 tcaaccgcta cgctcctggc gcgaaactgt cgctgcatca ggataaatct gaaccggatc
      481 tgcgcgcgcc aattgtttct gtttctctgg gcttacccgc gatttttcaa tttggcggcc
      541 tgaaacgtaa tgatccgctc aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg
      601 gcggtgaatc gcggctgttt tatcacggta ttcaaccgtt gaaagcgggg tttcatccac
      661 tcaccatcga ctgccgctac aacctgacat tccgtcaggc aggtaaaaaa gaataactcg
      721 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgaat
      781 tggctgctgc caccgctgag caataactag cataacc
//
