LOCUS       sequence-152943-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses super ecliptic A227D in mammalian cell membranes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Kang BE, Baker BJ
  TITLE     Pado, a fluorescent protein with proton channel activity can 
            optically monitor membrane potential, intracellular pH, and map gap 
            junctions.
  JOURNAL   Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865.
  PUBMED    27040905
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-4"; volume: "6:23865. doi"; pages: " 
            10.1038/srep23865"
COMMENT     Sequence Label: Farnesylated SE pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(100..813)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(140..160)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     755..783
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(901..920)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(902..920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggccctcta gactcgagct attaggagag cacacacttg cagctcatgc agccggggcc
       61 actctcatca ggagggttca gcttagatct gagtccggat ttgtatagtt catccatgcc
      121 atgtgtaatc ccatcagctg ttacaaactc aagaaggacc atgtggtctc tcttttcgtt
      181 gggatctttc gaaagagtag aagttgtaaa caggtaatgg ttgtctggta aaaggacggg
      241 cccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc ctccatcttc
      301 aatgttgtgt ctaattttga agttagcttt gattccattc ttttgtttgt ctgccatgat
      361 gtacacctgg tgatcgttat agttgtattc caatttgtgt ccaagaatgt ttccatcttc
      421 tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt caaacttgac
      481 ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt cctgtacata
      541 accttcgggc atggcactct tgaaaaagtc atgccgtttc atatgatctg ggtatcttga
      601 aaagcattga acaccataag ttaaagtagt gacaagtgtt ggccatggaa caggtagttt
      661 tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac cttcaccctc
      721 tccactgaca gaaaatttgt gcccattaac atcaccatct aattcaacaa gaattgggac
      781 aactccagtg aaaagttctt ctcctttact catgcgatat ctgcagaatt ccaccacact
      841 ggactagtgg atccgagctc ggtaccaagc ttaagtttaa acgctagcca gcttgggtct
      901 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
      961 agagagct
//
