LOCUS       sequence-152949-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses BE2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
  TITLE     Programmable editing of a target base in genomic DNA without 
            double-stranded DNA cleavage.
  JOURNAL   Nature. 2016 Apr 20. doi: 10.1038/nature17946.
  PUBMED    27096365
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2016 Apr 20. doi: 10.1038/nature17946."
COMMENT     Sequence Label: pCMV-BE2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             75..761
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
ORIGIN
        1 tggtttagtg aaccgtcaga tccgctagag atccgcggcc gctaatacga ctcactatag
       61 ggagagccgc caccatgagc tcagagactg gcccagtggc tgtggacccc acattgagac
      121 ggcggatcga gccccatgag tttgaggtat tcttcgatcc gagagagctc cgcaaggaga
      181 cctgcctgct ttacgaaatt aattgggggg gccggcactc catttggcga catacatcac
      241 agaacactaa caagcacgtc gaagtcaact tcatcgagaa gttcacgaca gaaagatatt
      301 tctgtccgaa cacaaggtgc agcattacct ggtttctcag ctggagccca tgcggcgaat
      361 gtagtagggc catcactgaa ttcctgtcaa ggtatcccca cgtcactctg tttatttaca
      421 tcgcaaggct gtaccaccac gctgaccccc gcaatcgaca aggcctgcgg gatttgatct
      481 cttcaggtgt gactatccaa attatgactg agcaggagtc aggatactgc tggagaaact
      541 ttgtgaatta tagcccgagt aatgaagccc actggcctag gtatccccat ctgtgggtac
      601 gactgtacgt tcttgaactg tactgcatca tactgggcct gcctccttgt ctcaacattc
      661 tgagaaggaa gcagccacag ctgacattct ttaccatcgc tcttcagtct tgtcattacc
      721 agcgactgcc cccacacatt ctctgggcca ccgggttgaa aagcggcagc gagactcccg
      781 ggacctcaga gtccgccaca cccgaaagtg ataaaaagta ttctattggt ttagccatcg
      841 gcactaattc cgttggatgg gctgtcataa ccgatgaata caa
//
