LOCUS       sequence-152952-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, 
            Weichenrieder O, Izaurralde E
  TITLE     Molecular architecture of 4E-BP translational inhibitors bound to 
            eIF4E.
  JOURNAL   Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 
            10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19.
  PUBMED    25702871
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.017"; journalName: "Mol Cell"; date: 
            "2015-03-19- 19"; volume: "57"; issue: "6"; pages: "1074-87"
COMMENT     Sequence Label: pLNHA-C1-Hs4E-BP3
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             104..169
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             194..220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    668..789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(705..724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     759..778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(933..954)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag ctctagcgct accggtcgcc accatggacg cacaaacacg
      121 acgacgtgag cgtcgcgctg agaaacaagc tcaatggaaa gctgcaaacg gtggatctcc
      181 tccacatatg gcttacccat acgatgttcc agattacgct cctccatctc gagctatgtc
      241 aacgtccact agctgcccga ttcccggggg ccgggaccag ctgcccgact gctacagcac
      301 cacgccgggg ggcacgctat acgccactac ccccggaggc accaggatca tctacgaccg
      361 aaagttcctg ctggagtgca agaactcacc cattgcccgg acacccccct gctgcctccc
      421 tcagattccc ggggtcacaa ctcctccaac agcccctctc tccaagctgg aggagctgaa
      481 ggagcaggag acagaggaag agatacccga tgacgcacaa tttgaaatgg acatctaagg
      541 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
      601 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
      661 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
      721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
      781 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
      841 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
      901 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
      961 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcaggg
//
