LOCUS       sequence-152958-        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged human 4E-BP3 in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Peter D, Igreja C, Weber R, Wohlbold L, Weiler C, Ebertsch L, 
            Weichenrieder O, Izaurralde E
  TITLE     Molecular architecture of 4E-BP translational inhibitors bound to 
            eIF4E.
  JOURNAL   Mol Cell. 2015 Mar 19;57(6):1074-87. doi: 
            10.1016/j.molcel.2015.01.017. Epub 2015 Feb 19.
  PUBMED    25702871
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.01.017"; journalName: "Mol Cell"; date: 
            "2015-03-19- 19"; volume: "57"; issue: "6"; pages: "1074-87"
COMMENT     Sequence Label: pLNHA-C1-Hs4E-BP3
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(465..491)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(516..581)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      578..587
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(614..633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(615..633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(678..881)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(707..727)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
       61 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
      121 atcagttatc tagatccggt ggatccttag atgtccattt caaattgtgc gtcatcgggt
      181 atctcttcct ctgtctcctg ctccttcagc tcctccagct tggagagagg ggctgttgga
      241 ggagttgtga ccccgggaat ctgagggagg cagcaggggg gtgtccgggc aatgggtgag
      301 ttcttgcact ccagcaggaa ctttcggtcg tagatgatcc tggtgcctcc gggggtagtg
      361 gcgtatagcg tgccccccgg cgtggtgctg tagcagtcgg gcagctggtc ccggcccccg
      421 ggaatcgggc agctagtgga cgttgacata gctcgagatg gaggagcgta atctggaaca
      481 tcgtatgggt aagccatatg tggaggagat ccaccgtttg cagctttcca ttgagcttgt
      541 ttctcagcgc gacgctcacg tcgtcgtgtt tgtgcgtcca tggtggcgac cggtagcgct
      601 agagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta agcagtgggt
      661 tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg cctaccgccc
      721 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
      781 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
      841 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
      901 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
      961 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     1021 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatgg
//
