LOCUS       sequence-152961-       17872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17872)
  AUTHORS   Hanaoka K, Qian F, Boletta A, Bhunia AK, Piontek K, Tsiokas L, 
            Sukhatme VP, Guggino WB, Germino GG
  TITLE     Co-assembly of polycystin-1 and -2 produces unique cation-permeable 
            currents.
  JOURNAL   Nature.   . 408(6815):990-4.
  PUBMED    11140688
REFERENCE   2  (bases 1 to 17872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature.   .
            408(6815):990-4."
COMMENT     Sequence Label: PKD1-F (pCI-PKD1-Flag)
FEATURES             Location/Qualifiers
     source          1..17872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(9..27)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        95..199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(200..268,269..1060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(418..437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1231..1819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1720..1739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2054..2433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2434..2637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2634..2658
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2773..2905
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2888..2907
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        2950..2968
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          2999..3571
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3579..3598
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             16742..16765
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    16825..16946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16836..16855)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     16890..16909
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      17180..17635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(17267..17286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17477..17498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(17702..17721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     17821..17843
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
       61 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      121 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      181 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      241 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      301 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      361 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      421 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      481 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      541 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      601 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      661 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      721 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      781 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      841 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      901 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      961 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1021 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1081 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1141 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1201 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1261 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1321 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1381 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1861 ctggcctttt gctcacatgg ctcgacagat ctggccaaaa aggccggacc ggatcttcaa
     1921 tattggccat tagccatatt attcattggt tatatagcat aaatcaatat tggctattgg
     1981 ccattgcata cgttgtatct atatcataat atgtacattt atattggctc atgtccaata
     2041 tgaccgccat gttggcattg attattgact agttattaat agtaatcaat tacggggtca
     2101 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     2161 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     2221 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     2281 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
     2341 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
     2401 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
     2461 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     2521 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc
     2581 ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     2641 ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag ttaaattgct
     2701 aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
     2761 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
     2821 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
     2881 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
     2941 tagagtactt aatacgactc actataggct agtctggatc gatcctgaga acttcagggt
     3001 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     3061 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     3121 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     3181 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     3241 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     3301 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     3361 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     3421 tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg
     3481 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     3541 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     3601 gtctcatcat tttggcaaag aattcgcggc cgcgtcgaca gcgtccgagc gggcggccga
     3661 gctcccggag cggcctggcc ccgagccccg agcgggcgtc gctcagcagc aggtcgcggc
     3721 cgcagcccca tccagccccg cgcccgccat gccgtccgcg ggccccgcct gagctgcggc
     3781 ctccgcgcgc gggcgggcct ggggacggcg gggccatgcg cgcgctgccc taacgatgcc
     3841 gcccgccgcg cccgcccgcc tggcgctggc cctgggcctg ggcctgtggc tcggggcgct
     3901 ggcggggggc cccgggcgcg gctgcgggcc ctgcgagccc ccctgcctct gcggcccagc
     3961 gcccggcgcc gcctgccgcg tcaactgctc gggccgcggg ctgcggacgc tcggtcccgc
     4021 gctgcgcatc cccgcggacg ccacagcgct agacgtctcc cacaacctgc tccgggcgct
     4081 ggacgttggg ctcctggcga acctctcggc gctggcagag ctggatataa gcaacaacaa
     4141 gatttctacg ttagaagaag gaatatttgc taatttattt aatttaagtg aaataaacct
     4201 gagtgggaac ccgtttgagt gtgactgtgg cctggcgtgg ctgccgcgat gggcggagga
     4261 gcagcaggtg cgggtggtgc agcccgaggc agccacgtgt gctgggcctg gctccctggc
     4321 tggccagcct ctgcttggca tccccttgct ggacagtggc tgtggtgagg agtatgtcgc
     4381 ctgcctccct gacaacagct caggcaccgt ggcagcagtg tccttttcag ctgcccacga
     4441 aggcctgctt cagccagagg cctgcagcgc cttctgcttc tccaccggcc agggcctcgc
     4501 agccctctcg gagcagggct ggtgcctgtg tggggcggcc cagccctcca gtgcctcctt
     4561 tgcctgcctg tccctctgct ccggcccccc gccacctcct gcccccacct gtaggggccc
     4621 caccctcctc cagcacgtct tccctgcctc cccaggggcc accctggtgg ggccccacgg
     4681 acctctggcc tctggccagc tagcagcctt ccacatcgct gccccgctcc ctgtcactgc
     4741 cacacgctgg gacttcggag acggctccgc cgaggtggat gccgctgggc cggctgcctc
     4801 gcatcgctat gtgctgcctg ggcgctatca cgtgacggcc gtgctggccc tgggggccgg
     4861 ctcagccctg ctggggacag acgtgcaggt ggaagcggca cctgccgccc tggagctcgt
     4921 gtgcccgtcc tcggtgcaga gtgacgagag ccttgacctc agcatccaga accgcggtgg
     4981 ttcaggcctg gaggccgcct acagcatcgt ggccctgggc gaggagccgg cccgagcggt
     5041 gcacccgctc tgcccctcgg acacggagat cttccctggc aacgggcact gctaccgcct
     5101 ggtggtggag aaggcggcct ggctgcaggc gcaggagcag tgtcaggcct gggccggggc
     5161 cgccctggca atggtggaca gtcccgccgt gcagcgcttc ctggtctctc gggtcaccag
     5221 gagcctagac gtgtggatcg gcttctcgac tgtgcagggg gtggaggtgg gcccagcgcc
     5281 gcagggcgag gccttcagcc tggagagctg ccagaactgg ctgcccgggg agccacaccc
     5341 agccacagcc gagcactgcg tccggctcgg gcccaccggg tggtgtaaca ccgacctgtg
     5401 ctcagcgccg cacagctacg tctgcgagct gcagcccgga ggcccagtgc aggatgccga
     5461 gaacctcctc gtgggagcgc ccagtgggga cctgcaggga cccctgacgc ctctggcaca
     5521 gcaggacggc ctctcagccc cgcacgagcc cgtggaggtc atggtattcc cgggcctgcg
     5581 tctgagccgt gaagccttcc tcaccacggc cgaatttggg acccaggagc tccggcggcc
     5641 cgcccagctg cggctgcagg tgtaccggct cctcagcaca gcagggaccc cggagaacgg
     5701 cagcgagcct gagagcaggt ccccggacaa caggacccag ctggcccccg cgtgcatgcc
     5761 agggggacgc tggtgccctg gagccaacat ctgcttgccg ctggacgcct cctgccaccc
     5821 ccaggcctgc gccaatggct gcacgtcagg gccagggcta cccggggccc cctatgcgct
     5881 atggagagag ttcctcttct ccgttcccgc ggggcccccc gcgcagtact cggtcaccct
     5941 ccacggccag gatgtcctca tgctccctgg tgacctcgtt ggcttgcagc acgacgctgg
     6001 ccctggcgcc ctcctgcact gctcgccggc tcccggccac cctggtcccc aggccccgta
     6061 cctctccgcc aacgcctcgt catggctgcc ccacttgcca gcccagctgg agggcacttg
     6121 ggcctgccct gcctgtgccc tgcggctgct tgcagccacg gaacagctca ccgtgctgct
     6181 gggcttgagg cccaaccctg gactgcggct gcctgggcgc tatgaggtcc gggcagaggt
     6241 gggcaatggc gtgtccaggc acaacctctc ctgcagcttt gacgtggtct ccccagtggc
     6301 tgggctgcgg gtcatctacc ctgccccccg cgacggccgc ctctacgtgc ccaccaacgg
     6361 ctcagccttg gtgctccagg tggactctgg tgccaacgcc acggccacgg ctcgctggcc
     6421 tgggggcagt gtcagcgccc gctttgagaa tgtctgccct gccctggtgg ccaccttcgt
     6481 gcccggctgc ccctgggaga ccaacgatac cctgttctca gtggtagcac tgccgtggct
     6541 cagtgagggg gagcacgtgg tggacgtggt ggtggaaaac agcgccagcc gggccaacct
     6601 cagcctgcgg gtgacggcgg aggagcccat ctgtggcctc cgcgccacgc ccagccccga
     6661 ggcccgtgta ctgcagggag tcctagtgag gtacagcccc gtggtggagg ccggctcgga
     6721 catggtcttc cggtggacca tcaacgacaa gcagtccctg accttccaga acgtggtctt
     6781 caatgtcatt tatcagagcg cggcggtctt caagctctca ctgacggccc ccaaccacgt
     6841 gagcaacgtc accgtgaact acaacgtaac cgtggagcgg atgaacagga tgcagggtct
     6901 gcaggtctcc acagtgccgg ccgtgctgtc ccccaatgcc acgctggcac tgacggcggg
     6961 cgtgctggtg gactcggccg tggaggtggc cttcctgtgg acctttgggg atggggagca
     7021 ggccctccac cagttccagc ctccgtacaa cgagtccttc ccggttccag acccctcggt
     7081 ggcccaggtg ctggtggagc acaatgtcat gcacacctac gctgccccag gtgagtacct
     7141 cctgaccgtg ctggcatcta atgccttcga gaacctgacg cagcaggtgc ctgtgagcgt
     7201 gcgcgcctcc ctgccctccg tggctgtggg tgtgagtgac ggcgtcctgg tggccggccg
     7261 gcccgtcacc ttctacccgc acccgctgcc ctcgcctggg ggtgttcttt acacgtggga
     7321 cttcggggac ggctcccctg tcctgaccca gagccagccg gctgccaacc acacctatgc
     7381 ctcgaggggc acctaccacg tgcgcctgga ggtcaacaac acggtgagcg gtgcggcggc
     7441 ccaggcggat gtgcgcgtct ttgaggagct ccgcggactc agcgtggaca tgagcctggc
     7501 cgtggagcag ggcgcccccg tggtggtcag cgccgcggtg cagacgggcg acaacatcac
     7561 gtggaccttc gacatggggg acggcaccgt gctgtcgggc ccggaggcaa cagtggagca
     7621 tgtgtacctg cgggcacaga actgcacagt gaccgtgggt gcggccagcc ccgccggcca
     7681 cctggcccgg agcctgcacg tgctggtctt cgtcctggag gtgctgcgcg ttgaacccgc
     7741 cgcctgcatc cccacgcagc ctgacgcgcg gctcacggcc tacgtcaccg ggaacccggc
     7801 ccactacctc ttcgactgga ccttcgggga tggctcctcc aacacgaccg tgcgggggtg
     7861 cccgacggtg acacacaact tcacgcggag cggcacgttc cccctggcgc tggtgctgtc
     7921 cagccgcgtg aacagggcgc attacttcac cagcatctgc gtggagccag aggtgggcaa
     7981 cgtcaccctg cagccagaga ggcagtttgt gcagctcggg gacgaggcct ggctggtggc
     8041 atgtgcctgg cccccgttcc cctaccgcta cacctgggac tttggcaccg aggaagccgc
     8101 ccccacccgt gccaggggcc ctgaggtgac gttcatctac cgagacccag gctcctatct
     8161 tgtgacagtc accgcgtcca acaacatctc tgctgccaat gactcagccc tggtggaggt
     8221 gcaggagccc gtgctggtca ccagcatcaa ggtcaatggc tcccttgggc tggagctgca
     8281 gcagccgtac ctgttctctg ctgtgggccg tgggcgcccc gccagctacc tgtgggatct
     8341 gggggacggt gggtggctcg agggtccgga ggtcacccac gcttacaaca gcacaggtga
     8401 cttcaccgtt agggtggccg gctggaatga ggtgagccgc agcgaggcct ggctcaatgt
     8461 gacggtgaag cggcgcgtgc gggggctcgt cgtcaatgca agccgcacgg tggtgcccct
     8521 gaatgggagc gtgagcttca gcacgtcgct ggaggccggc agtgatgtgc gctattcctg
     8581 ggtgctctgt gaccgctgca cgcccatccc tgggggtcct accatctctt acaccttccg
     8641 ctccgtgggc accttcaata tcatcgtcac ggctgagaac gaggtgggct ccgcccagga
     8701 cagcatcttc gtctatgtcc tgcagctcat agaggggctg caggtggtgg gcggtggccg
     8761 ctacttcccc accaaccaca cggtacagct gcaggccgtg gttagggatg gcaccaacgt
     8821 ctcctacagc tggactgcct ggagggacag gggcccggcc ctggccggca gcggcaaagg
     8881 cttctcgctc accgtgctcg aggccggcac ctaccatgtg cagctgcggg ccaccaacat
     8941 gctgggcagc gcctgggccg actgcaccat ggacttcgtg gagcctgtgg ggtggctgat
     9001 ggtggccgcc tccccgaacc cagctgccgt caacacaagc gtcaccctca gtgccgagct
     9061 ggctggtggc agtggtgtcg tatacacttg gtccttggag gaggggctga gctgggagac
     9121 ctccgagcca tttaccaccc atagcttccc cacacccggc ctgcacttgg tcaccatgac
     9181 ggcagggaac ccgctgggct cagccaacgc caccgtggaa gtggatgtgc aggtgcctgt
     9241 gagtggcctc agcatcaggg ccagcgagcc cggaggcagc ttcgtggcgg ccgggtcctc
     9301 tgtgcccttt tgggggcagc tggccacggg caccaatgtg agctggtgct gggctgtgcc
     9361 cggcggcagc agcaagcgtg gccctcatgt caccatggtc ttcccggatg ctggcacctt
     9421 ctccatccgg ctcaatgcct ccaacgcagt cagctgggtc tcagccacgt acaacctcac
     9481 ggcggaggag cccatcgtgg gcctggtgct gtgggccagc agcaaggtgg tggcgcccgg
     9541 gcagctggtc cattttcaga tcctgctggc tgccggctca gctgtcacct tccgcctgca
     9601 ggtcggcggg gccaaccccg aggtgctccc cgggccccgt ttctcccaca gcttcccccg
     9661 cgtcggagac cacgtggtga gcgtgcgggg caaaaaccac gtgagctggg cccaggcgca
     9721 ggtgcgcatc gtggtgctgg aggccgtgag tgggctgcag gtgcccaact gctgcgagcc
     9781 tggcatcgcc acgggcactg agaggaactt cacagcccgc gtgcagcgcg gctctcgggt
     9841 cgcctacgcc tggtacttct cgctgcagaa ggtccagggc gactcgctgg tcatcctgtc
     9901 gggccgcgac gtcacctaca cgcccgtggc cgcggggctg ttggagatcc aggtgcgcgc
     9961 cttcaacgcc ctgggcagtg agaaccgcac gctggtgctg gaggttcagg acgccgtcca
    10021 gtatgtggcc ctgcagagcg gcccctgctt caccaaccgc tcggcgcagt ttgaggccgc
    10081 caccagcccc agcccccggc gtgtggccta ccactgggac tttggggatg ggtcgccagg
    10141 gcaggacaca gatgagccca gggccgagca ctcctacctg aggcctgggg actaccgcgt
    10201 gcaggtgaac gcctccaacc tggtgagctt cttcgtggcg caggccacgg tgaccgtcca
    10261 ggtgctggcc tgccgggagc cggaggtgga cgtggtcctg cccctgcagg tgctgatgcg
    10321 gcgatcacag cgcaactact tggaggccca cgttgacctg cgcgactgcg tcacctacca
    10381 gactgagtac cgctgggagg tgtatcgcac cgccagctgc cagcggccgg ggcgcccagc
    10441 gcgtgtggcc ctgcccggcg tggacgtgag ccggcctcgg ctggtgctgc cgcggctggc
    10501 gctgcctgtg gggcactact gctttgtgtt tgtcgtgtca tttggggaca cgccactgac
    10561 acagagcatc caggccaatg tgacggtggc ccccgagcgc ctggtgccca tcattgaggg
    10621 tggctcatac cgcgtgtggt cagacacacg ggacctggtg ctggatggga gcgagtccta
    10681 cgaccccaac ctggaggacg gcgaccagac gccgctcagt ttccactggg cctgtgtggc
    10741 ttcgacacag agggaggctg gcgggtgtgc gctgaacttt gggccccgcg ggagcagcac
    10801 ggtcaccatt ccacgggagc ggctggcggc tggcgtggag tacaccttca gcctgaccgt
    10861 gtggaaggcc ggccgcaagg aggaggccac caaccagacg gtgctgatcc ggagtggccg
    10921 ggtgcccatt gtgtccttgg agtgtgtgtc ctgcaaggca caggccgtgt acgaagtgag
    10981 ccgcagctcc tacgtgtact tggagggccg ctgcctcaat tgcagcagcg gctccaagcg
    11041 agggcggtgg gctgcacgta cgttcagcaa caagacgctg gtgctggatg agaccaccac
    11101 atccacgggc agtgcaggca tgcgactggt gctgcggcgg ggcgtgctgc gggacggcga
    11161 gggatacacc ttcacgctca cggtgctggg ccgctctggc gaggaggagg gctgcgcctc
    11221 catccgcctg tcccccaacc gcccgccgct ggggggctct tgccgcctct tcccactggg
    11281 cgctgtgcac gccctcacca ccaaggtgca cttcgaatgc acgggctggc atgacgcgga
    11341 ggatgctggc gccccgctgg tgtacgccct gctgctgcgg cgctgtcgcc agggccactg
    11401 cgaggagttc tgtgtctaca agggcagcct ctccagctac ggagccgtgc tgcccccggg
    11461 tttcaggcca cacttcgagg tgggcctggc cgtggtggtg caggaccagc tgggagccgc
    11521 tgtggtcgcc ctcaacaggt ctttggccat caccctccca gagcccaacg gcagcgcaac
    11581 ggggctcaca gtctggctgc acgggctcac cgctagtgtg ctcccagggc tgctgcggca
    11641 ggccgatccc cagcacgtca tcgagtactc gttggccctg gtcaccgtgc tgaacgagta
    11701 cgagcgggcc ctggacgtgg cggcagagcc caagcacgag cggcagcacc gagcccagat
    11761 acgcaagaac atcacggaga ctctggtgtc cctgagggtc cacactgtgg atgacatcca
    11821 gcagatcgct gctgcgctgg cccagtgcat ggggcccagc agggagctcg tatgccgctc
    11881 gtgcctgaag cagacgctgc acaagctgga ggccatgatg ctcatcctgc aggcagagac
    11941 caccgcgggc accgtgacgc ccaccgccat cggagacagc atcctcaaca tcacaggaga
    12001 cctcatccac ctggccagct cggacgtgcg ggcaccacag ccctcagagc tgggagccga
    12061 gtcaccatct cggatggtgg cgtcccaggc ctacaacctg acctctgccc tcatgcgcat
    12121 cctcatgcgc tcccgcgtgc tcaacgagga gcccctgacg ctggcgggcg aggagatcgt
    12181 ggcccagggc aagcgctcgg acccgcggag cctgctgtgc tatggcggcg ccccagggcc
    12241 tggctgccac ttctccatcc ccgaggcttt cagcggggcc ctggccaacc tcagtgacgt
    12301 ggtgcagctc atctttctgg tggactccaa tccctttccc tttggctata tcagcaacta
    12361 caccgtctcc accaaggtgg cctcgatggc attccagaca caggccggcg cccagatccc
    12421 catcgagcgg ctggcctcag agcgcgccat caccgtgaag gtgcccaaca actcggactg
    12481 ggctgcccgg ggccaccgca gctccgccaa ctccgccaac tccgttgtgg tccagcccca
    12541 ggcctccgtc ggtgctgtgg tcaccctgga cagcagcaac cctgcggccg ggctgcatct
    12601 gcagctcaac tatacgctgc tggacggcca ctacctgtct gaggaacctg agccctacct
    12661 ggcagtctac ctacactcgg agccccggcc caatgagcac aactgctcgg ctagcaggag
    12721 gatccgccca gagtcactcc agggtgctga ccaccggccc tacaccttct tcatttcccc
    12781 ggggagcaga gacccagcgg ggagttacca tctgaacctc tccagccact tccgctggtc
    12841 ggcgctgcag gtgtccgtgg gcctgtacac gtccctgtgc cagtacttca gcgaggagga
    12901 catggtgtgg cggacagagg ggctgctgcc cctggaggag acctcgcccc gccaggccgt
    12961 ctgcctcacc cgccacctca ccgccttcgg cgccagcctc ttcgtgcccc caagccatgt
    13021 ccgctttgtg tttcctgagc cgacagcgga tgtaaactac atcgtcatgc tgacatgtgc
    13081 tgtgtgcctg gtgacctaca tggtcatggc cgccatcctg cacaagctgg accagttgga
    13141 tgccagccgg ggccgcgcca tccctttctg tgggcagcgg ggccgcttca agtacgagat
    13201 cctcgtcaag acaggctggg gccggggctc aggtaccacg gcccacgtgg gcatcatgct
    13261 gtatggggtg gacagccgga gcggccaccg gcacctggac ggcgacagag ccttccaccg
    13321 caacagcctg gacatcttcc ggatcgccac cccgcacagc ctgggtagcg tgtggaagat
    13381 ccgagtgtgg cacgacaaca aagggctcag ccctgcctgg ttcctgcagc acgtcatcgt
    13441 cagggacctg cagacggcac gcagcgcctt cttcctggtc aatgactggc tttcggtgga
    13501 gacggaggcc aacgggggcc tggtggagaa ggaggtgctg gccgcgagcg acgcagccct
    13561 tttgcgcttc cggcgcctgc tggtggctga gctgcagcgt ggcttctttg acaagcacat
    13621 ctggctctcc atatgggacc ggccgcctcg tagccgtttc actcgcatcc agagggccac
    13681 ctgctgcgtt ctcctcatct gcctcttcct gggcgccaac gccgtgtggt acggggctgt
    13741 tggcgactct gcctacagca cggggcatgt gtccaggctg agcccgctga gcgtcgacac
    13801 agtcgctgtt ggcctggtgt ccagcgtggt tgtctatccc gtctacctgg ccatcctttt
    13861 tctcttccgg atgtcccgga gcaaggtggc tgggagcccg agccccacac ctgccgggca
    13921 gcaggtgctg gacatcgaca gctgcctgga ctcgtccgtg ctggacagct ccttcctcac
    13981 gttctcaggc ctccacgctg aggcctttgt tggacagatg aagagtgact tgtttctgga
    14041 tgattctaag agtctggtgt gctggccctc cggcgaggga acgctcagtt ggccggacct
    14101 gctcagtgac ccgtccattg tgggtagcaa tctgcggcag ctggcacggg gccaggcggg
    14161 ccatgggctg ggcccagagg aggacggctt ctccctggcc agcccctact cgcctgccaa
    14221 atccttctca gcatcagatg aagacctgat ccagcaggtc cttgccgagg gggtcagcag
    14281 cccagcccct acccaagaca cccacatgga aacggacctg ctcagcagcc tgtccagcac
    14341 tcctggggag aagacagaga cgctggcgct gcagaggctg ggggagctgg ggccacccag
    14401 cccaggcctg aactgggaac agccccaggc agcgaggctg tccaggacag gactggtgga
    14461 gggtctgcgg aagcgcctgc tgccggcctg gtgtgcctcc ctggcccacg ggctcagcct
    14521 gctcctggtg gctgtggctg tggctgtctc agggtgggtg ggtgcgagct tccccccggg
    14581 cgtgagtgtt gcgtggctcc tgtccagcag cgccagcttc ctggcctcat tcctcggctg
    14641 ggagccactg aaggtcttgc tggaagccct gtacttctca ctggtggcca agcggctgca
    14701 cccggatgaa gatgacaccc tggtagagag cccggctgtg acgcctgtga gcgcacgtgt
    14761 gccccgcgta cggccacccc acggctttgc actcttcctg gccaaggaag aagcccgcaa
    14821 ggtcaagagg ctacatggca tgctgcggag cctcctggtg tacatgcttt ttctgctggt
    14881 gaccctgctg gccagctatg gggatgcctc atgccatggg cacgcctacc gtctgcaaag
    14941 cgccatcaag caggagctgc acagccgggc cttcctggcc atcacgcggt ctgaggagct
    15001 ctggccatgg atggcccacg tgctgctgcc ctacgtccac gggaaccagt ccagcccaga
    15061 gctggggccc ccacggctgc ggcaggtgcg gctgcaggaa gcactctacc cagaccctcc
    15121 cggccccagg gtccacacgt gctcggccgc aggaggcttc agcaccagcg attacgacgt
    15181 tggctgggag agtcctcaca atggctcggg gacgtgggcc tattcagcgc cggatctgct
    15241 gggggcatgg tcctggggct cctgtgccgt gtatgacagc gggggctacg tgcaggagct
    15301 gggcctgagc ctggaggaga gccgcgaccg gctgcgcttc ctgcagctgc acaactggct
    15361 ggacaacagg agccgcgctg tgttcctgga gctcacgcgc tacagcccgg ccgtggggct
    15421 gcacgccgcc gtcacgctgc gcctcgagtt cccggcggcc ggccgcgccc tggccgccct
    15481 cagcgtccgc ccctttgcgc tgcgccgcct cagcgcgggc ctctcgctgc ctctgctcac
    15541 ctcggtgtgc ctgctgctgt tcgccgtgca cttcgccgtg gccgaggccc gtacttggca
    15601 cagggaaggg cgctggcgcg tgctgcggct cggagcctgg gcgcggtggc tgctggtggc
    15661 gctgacggcg gccacggcac tggtacgcct cgcccagctg ggtgccgctg accgccagtg
    15721 gacccgtttc gtgcgcggcc gcccgcgccg cttcactagc ttcgaccagg tggcgcagct
    15781 gagctccgca gcccgtggcc tggcggcctc gctgctcttc ctgcttttgg tcaaggctgc
    15841 ccagcagcta cgcttcgtgc gccagtggtc cgtctttggc aagacattat gccgagctct
    15901 gccagagctc ctgggggtca ccttgggcct ggtggtgctc ggggtagcct acgcccagct
    15961 ggccatcctg ctcgtgtctt cctgtgtgga ctccctctgg agcgtggccc aggccctgtt
    16021 ggtgctgtgc cctgggactg ggctctctac cctgtgtcct gccgagtcct ggcacctgtc
    16081 acccctgctg tgtgtggggc tctgggcact gcggctgtgg ggcgccctac ggctgggggc
    16141 tgttattctc cgctggcgct accacgcctt gcgtggagag ctgtaccggc cggcctggga
    16201 gccccaggac tacgagatgg tggagttgtt cctgcgcagg ctgcgcctct ggatgggcct
    16261 cagcaaggtc aaggagttcc gccacaaagt ccgctttgaa gggatggagc cgctgccctc
    16321 tcgctcctcc aggggctcca aggtatcccc ggatgtgccc ccacccagcg ctggctccga
    16381 tgcctcgcac ccctccacct cctccagcca gctggatggg ctgagcgtga gcctgggccg
    16441 gctggggaca aggtgtgagc ctgagccctc ccgcctccaa gccgtgttcg aggccctgct
    16501 cacccagttt gaccgactca accaggccac agaggacgtc taccagctgg agcagcagct
    16561 gcacagcctg caaggccgca ggagcagccg ggcgcccgcc ggatcttccc gtggcccatc
    16621 cccgggcctg cggccagcac tgcccagccg ccttgcccgg gccagtcggg gtgtggacct
    16681 ggccactggc cccagcagga caccccttcg ggccaagaac aaggtccacc ccagcagcac
    16741 tgactacaag gacgacgatg acaagtgatc aggtagctta tcgataccgt cgacccgggc
    16801 ggccgccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
    16861 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
    16921 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
    16981 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
    17041 tcgataagga tcaattgcct ttttttttaa tttttatttt attttatttt tgagatggcc
    17101 agatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    17161 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    17221 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    17281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    17341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    17401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    17461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    17521 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    17581 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
    17641 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    17701 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    17761 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    17821 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg ag
//
