LOCUS       sequence-152963-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human DNMT1 with myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Li H, Rauch T, Chen ZX, Szabo PE, Riggs AD, Pfeifer GP
  TITLE     The histone methyltransferase SETDB1 and the DNA methyltransferase 
            DNMT3A interact directly and localize to promoters silenced in 
            cancer cells.
  JOURNAL   J Biol Chem. 2006 Jul 14;281(28):19489-500. Epub 2006 May 8.
  PUBMED    16682412
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-07-14"; volume: "281(28)"; pages: "19489-500. 
            Epub 2006 May 8"
COMMENT     Sequence Label: pcDNA3/Myc-DNMT1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             77..106
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttgcc accatggagc agaagctgat ctcagaggag gacctggaat tcatgccggc
      121 gcgtaccgcc ccagcccggg tgcccacact ggccgtcccg gccatctcgc tgcccgacga
      181 tgtccgcagg cggctcaaag atttggaaag agacagctta acagaaaagg aatgtgtgaa
      241 ggagaaattg aatctcttgc acgaatttct gcaaacagaa ataaagaatc agttatgtga
      301 cttggaaacc aaattacgta aagaagaatt atccgaggag ggctacctgg ctaaagtcaa
      361 atccctttta aataaagatt tgtccttgga gaacggtgct catgcttaca accgggaagt
      421 gaatggacgt ctagaaaacg ggaaccaagc aagaagtgaa gcccgtagag tgggaatggc
      481 agatgccaac agccccccca aacccctttc caaacctcgc acgcccagga ggagcaagtc
      541 cgatggagag gctaagcctg aaccttcacc tagccccagg attacaagga aaagcaccag
      601 gcaaaccacc atcacatctc attttgcaaa gggccctgcc aaacggaaac ctcaggaaga
      661 gtctgaaaga gccaaatcgg atgagtccat caaggaagaa gacaaagacc aggatgagaa
      721 gagacgtaga gttacatcca gagaacgagt tgctagaccg cttcctgcag aagaacctga
      781 aagagcaaaa tcaggaacgc gcactgaaaa ggaagaaga
//
