LOCUS       sequence-152965-        1751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Chen Lf , Fischle W, Verdin E, Greene WC
  TITLE     Duration of nuclear NF-kappaB action regulated by reversible 
            acetylation.
  JOURNAL   Science. 2001 Aug 31. 293(5535):1653-7.
  PUBMED    11533489
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2001 Aug 31. 293(5535):1653-7."
COMMENT     Sequence Label: T7-RelA
FEATURES             Location/Qualifiers
     source          1..1751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             1354..1725
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
ORIGIN
        1 cttgtagaag cgcgtatggc ttcgtgggga tctatggcta gcatgactgg tggacagcaa
       61 atgggtcgcg gatccgacga actgttcccc ctcatcttcc cggcagagcc agcccaggcc
      121 tctggcccct atgtggagat cattgagcag cccaagcagc ggggcatgcg cttccgctac
      181 aagtgcgagg ggcgctccgc gggcagcatc ccaggcgaga ggagcacaga taccaccaag
      241 acccacccca ccatcaagat caatggctac acaggaccag ggacagtgcg catctccctg
      301 gtcaccaagg accctcctca ccggcctcac ccccacgagc ttgtaggaaa ggactgccgg
      361 gatggcttct atgaggctga gctctgcccg gaccgctgca tccacagttt ccagaacctg
      421 ggaatccagt gtgtgaagaa gcgggacctg gagcaggcta tcagtcagcg catccagacc
      481 aacaacaacc ccttccaagt tcctatagaa gagcagcgtg gggactacga cctgaatgct
      541 gtgcggctct gcttccaggt gacagtgcgg gacccatcag gcaggcccct ccgcctgccg
      601 cctgtccttt ctcatcccat ctttgacaat cgtgccccca acactgccga gctcaagatc
      661 tgccgagtga accgaaactc tggcagctgc ctcggtgggg atgagatctt cctactgtgt
      721 gacaaggtgc agaaagagga cattgaggtg tatttcacgg gaccaggctg ggaggcccga
      781 ggctcctttt cgcaagctga tgtgcaccga caagtggcca ttgtgttccg gacccctccc
      841 tacgcagacc ccagcctgca ggctcctgtg cgtgtctcca tgcagctgcg gcggccttcc
      901 gaccgggagc tcagtgagcc catggaattc cagtacctgc cagatacaga cgatcgtcac
      961 cggattgagg agaaacgtaa aaggacatat gagaccttca agagcatcat gaagaagagt
     1021 cctttcagcg gacccaccga cccccggcct ccacctcgac gcattgctgt gccttcccgc
     1081 agctcagctt ctgtccccaa gccagcaccc cagccctatc cctttacgtc atccctgagc
     1141 accatcaact atgatgagtt tcccaccatg gtgtttcctt ctgggcagat cagccaggcc
     1201 tcggccttgg ccccggcccc tccccaagtc ctgccccagg ctccagcccc tgcccctgct
     1261 ccagccatgg tatcagctct ggcccaggcc ccagcccctg tcccagtcct agccccaggc
     1321 cctcctcagg ctgtggcccc acctgccccc aagcccaccc aggctgggga aggaacgctg
     1381 tcagaggccc tgctgcagct gcagtttgat gatgaagacc tgggggcctt gcttggcaac
     1441 agcacagacc cagctgtgtt cacagacctg gcatccgtcg acaactccga gtttcagcag
     1501 ctgctgaacc agggcatacc tgtggccccc cacacaactg agcccatgct gatggagtac
     1561 cctgaggcta taactcgcct agtgacaggg gcccagaggc cccccgaccc agctcctgct
     1621 ccactggggg ccccggggct ccccaatggc ctcctttcag gagatgaaga cttctcctcc
     1681 attgcggaca tggacttctc agccctgctg agtcagatca gctcctaatc tagagctgag
     1741 aacttcaggg t
//
