LOCUS       sequence-152970-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe 
            Harbor Locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             68..88
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             107..172
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(182..187,188..211,212..247,248..271)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 ggctccctcg ttgaccgaat caccgacctc tctccccagg ggtaccctca gccttaaggc
       61 ggccgccgag aatctgtatt ttcagggagc catgggatcc gccggcgact acaaggacca
      121 cgacggcgat tataaggatc acgacatcga ctacaaagac gacgatgaca agggcgccag
      181 cagcgcctgg tcccaccctc agtttgagaa gggcggaggc tctggcggcg gaagcggagg
      241 atctgcttgg agccaccccc agttcgaaaa gggcgccggc cagactggga agaaatctga
      301 gaagggacca gtttgttggc ggaagcgtgt aaaatcagag tacatgcgac tgagacagct
      361 caagaggttc agacgagctg atgaagtaaa gagtatgttt agttccaatc gtcagaaaat
      421 tttggaaaga acggaaatct taaaccaaga atggaaacag cgaaggatac agcctgtgca
      481 catcctgact tctgtgagct cattgcgcgg gactagggag tgttcggtga ccagtgactt
      541 ggattttcca acacaagtca tcccattaaa gactctgaat gcagttgctt cagtacccat
      601 aatgtattct tggtctcccc tacagcagaa ttttatggtg gaagatgaaa ctgttttaca
      661 taacattcct tatatgggag atgaagtttt agatcaggat ggtactttca ttgaagaact
      721 aataaaaaat tatgatggga aagtacacgg ggatagagaa tgtgggttta taaatgatga
      781 aatttttgtg gagttggtga atgcccttgg tcaatataat gatgatgacg atgatgatga
      841 tggagacgat cctgaagaaa gagaagaaaa gcagaaagat ctggaggatc accgagatga
      901 taaagaaagc cgcccacctc ggaaatttcc ttctgataaa atttttgaag ccatttcctc
      961 aatgtttcca gataagggca cagcagaaga actaaaggaa aaatataaag aactcaccga
     1021 a
//
