LOCUS       sequence-152971-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe 
            Harbor Locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatggctggc aactagaagg cacagcctgc aggtcaaggg atttccattt ctctttcgat
       61 gccgacatac ttcagggcat cagcctggct gtatctgtaa tcaaaaaaca gctcttcgcc
      121 agtctggatg gctctcttgg caaaaatacc tatcctgtga tcaccgttaa ccatcataac
      181 ttttgcatag cagtttggat ttaccgaatg atttgcaaaa cgaattttgt tacccttgcg
      241 ggttgcatcc accacaaaat cattgttcaa gttgaacaga aagctgcaca tgtatttatc
      301 atacactttc cctcttctgt cagcttcatc ttgagaaata atctctccac agtattctga
      361 gatgaattca tttttctgca caggatcttt gataaaaatc ccccagcctg ccacgtcaga
      421 tggtgccagc aatagatgct ttttggagcc ccgctgaata ctgcagttct tgcaggacac
      481 atttttactg tcccaatggt cagcggctcc acaagtaaga cagaggtcag ggtcacactc
      541 tcggacagcc aggtagcacg ggcactgctt ggtgttgcac tgtgctttgc agcggcatcc
      601 cggaaagcgg ttttgacact ctgaactaca ttgacaaaac ttttcacaaa aattttgtgc
      661 tatcacacaa gggcacgaac tgtcacaagg ctgccgtgga tgatcacagg gttgatagtt
      721 gtaaacatgg ttagaggagc cgtccttttt cagctgtatc tttctgcagt gtgcagccca
      781 caaccggtgt ttcctcttct ttttccttgg aggagtatcc acatcctcag cgggagctgg
      841 agctatgatg ctagattctt tgactctaaa ctcatacacc tgtctacatg ttttggtccc
      901 aattaaccta gcaatggcac agaaattgtc atagtaagtg ccaatgagga ctctaaacat
      961 tgaggcttca gcaccactcc actcca
//
