LOCUS       sequence-152975-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP1-10 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHR-SFFV-GFP1-10
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             122..763
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag ctctcgagaa
       61 ttctcacgcg tctgcaggat atcaagcttg cggtaccgcg ggcccgggat ccgccgccac
      121 catgtccaaa ggagaagaac tgtttaccgg tgttgtgcca attttggttg aactcgatgg
      181 tgatgtcaac ggacataagt tctcagtgag aggcgaagga gaaggtgacg ccaccattgg
      241 aaaattgact cttaaattca tctgtactac tggtaaactt cctgtaccat ggccgactct
      301 cgtaacaacg cttacgtacg gagttcagtg cttttcgaga tacccagacc atatgaaaag
      361 acatgacttt tttaagtcgg ctatgcctga aggttacgtg caagaaagaa caatttcgtt
      421 caaagatgat ggaaaatata aaactagagc agttgttaaa tttgaaggag atactttggt
      481 taaccgcatt gaactgaaag gaacagattt taaagaagat ggtaatattc ttggacacaa
      541 actcgaatac aattttaata gtcataacgt atacatcact gctgataagc aaaagaacgg
      601 aattaaagcg aatttcacag tacgccataa tgtagaagat ggcagtgttc aacttgccga
      661 ccattaccaa caaaacaccc ctattggaga cggtccggta cttcttcctg ataatcacta
      721 cctctcaaca caaacagtcc tgagcaaaga tccaaatgaa aaataagcgg ccgcgactct
      781 agagtcgacc tgcagg
//
