LOCUS       sequence-152976-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the AAVS1 T2 gRNA in combination with FLAGless 
            eSpCas9(1.1) to target the AAVS1 ""safe harbor"" locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: eSpCas9(1.1)_No_FLAG_AAVS1_T2
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccggactgct tgtcccggat gccgttgatc agcttccggc tcagcctgcc ccagccggtg
       61 tatctccgcc gcttcagctg cttcatcact ttgtcgtcga acaggtgggc ataggttttc
      121 agccgttcct cgatcatctc tctgtcctca aacagtgtca gggtcagcac gatatcttcc
      181 agaatgtcct cgttttcctc attgtccagg aagtccttgt ccttgataat tttcagcaga
      241 tcgtggtatg tgcccaggga ggcgttgaac cgatcttcca cgccggagat ttccacggag
      301 tcgaagcact cgattttctt gaagtagtcc tctttcagct gcttcacggt cactttccgg
      361 ttggtcttga acagcaggtc cacgatggcc tttttctgct cgccgctcag gaaggcgggc
      421 tttctcattc cctcggtcac gtatttcact ttggtcagct cgttatacac ggtgaagtac
      481 tcgtacagca ggctgtgctt gggcagcacc ttctcgttgg gcaggttctt atcgaagttg
      541 gtcatccgct cgatgaagct ctgggcggaa gcgcccttgt ccaccacttc ctcgaagttc
      601 cagggggtga tggtttcctc gctctttctg gtcatccagg cgaatctgct gtttcccctg
      661 gccagagggc ccacgtagta ggggatgcgg aaggtcagga tcttctcgat cttttcccgg
      721 ttgtccttca ggaatgggta aaaatcttcc tgccgccgca gaatggcgtg cagctctccc
      781 aggtggatct ggtgggggat gctgccgttg tcgaaggtcc gctgcttccg cagcaggtcc
      841 tctctgttca gcttcacgag cagttcctcg gtgccgtcca tcttttccag gatgggcttg
      901 atgaacttgt agaactcttc ctggctggct ccgccgtcaa tgtagccggc gtagccgttc
      961 ttgctctggt cgaagaaaat ctctttgtac ttctcaggca gctgctgccg cacgagagct
     1021 t
//
