LOCUS       sequence-152979-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . 
            TPC2-GCaMP6 allows the visualization of Ca2+ release from 
            intracellular Ca2+ stores in real time.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Ambrosio AL, Boyle JA, Di Pietro SM
  TITLE     TPC2 mediates new mechanisms of platelet dense granule membrane 
            dynamics through regulation of Ca2+ release.
  JOURNAL   Mol Biol Cell. 2015 Sep 15;26(18):3263-74. doi: 
            10.1091/mbc.E15-01-0058. Epub 2015 Jul 22.
  PUBMED    26202466
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-01-0058"; journalName: "Mol Biol Cell"; date: 
            "2015-09-15- 15"; volume: "26"; issue: "18"; pages: "3263-74"
COMMENT     Sequence Label: pGCaMP6m-N3-TPC2
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(317..373)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     regulatory      385..394
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(join(401..415,416..424))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(428..460)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(464..481)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gctcctcgcc cttgctcacc atgctccctc cggtaccgcc cttgtacagc tcgtccatgc
       61 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
      121 tggggtcttt cgaaagtttg gactgcacgc tcaggtagtg gttgtcgggc agcagcacgg
      181 ggccgtcgcc gatgggggtg ttctgctggt agtggtaggc gagctgcacg ccgccgtcct
      241 cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg tcggccttga
      301 tatagacgtt ctcgagtgag ctcagccgac ctatagctct gactgcgtga cctgtcttat
      361 tccacttacg acgtgatgag tcgaccatgg tggcgagatc cttatcgtca tcgtcgtaca
      421 gatcccgacc catttgctgt ccaccagtca tgctagccat accatgatga tgatgatgat
      481 gagaacccat ggatcccggg cccgcggtac cgtcgactgc agaattctcc ctgcacagcc
      541 acaggtgcgg gtgctggctc agcctctctg tgagctcatc ctcctcgggc tcctccagaa
      601 tatccctgaa caggagctcc acagtcatct ggtaggtggc ctctggggtc ccagcaaggg
      661 gctgcaggtg gctgcggggg tcccacttgt gaaggaagtt ctccagaatc agggccagaa
      721 acaggttgac ccagatgaca gacgacacca gccaccacaa tacaaaatag atcttggacc
      781 acgggcctga gtagcgccga tatgcatcca
//
