LOCUS       sequence-152982-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the AAVS1 T2 gRNA in combination with FLAGless 
            eSpCas9(1.1) to target the AAVS1 ""safe harbor"" locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: eSpCas9(1.1)_No_FLAG_AAVS1_T2
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        119..367
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     119..139
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     290..309
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        388..463
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtga
      121 gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat
      181 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
      241 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      301 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      361 aaacaccggg gccactaggg acaggatgtt ttagagctag aaatagcaag ttaaaataag
      421 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg ttttagagct
      481 agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa ttctgcagac
      541 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      601 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
      661 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      721 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtg
//
