LOCUS       sequence-152984-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmMextli in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Peter D, Weber R, Kone C, Chung MY, Ebertsch L, Truffault V, 
            Weichenrieder O, Igreja C, Izaurralde E
  TITLE     Mextli proteins use both canonical bipartite and novel tripartite 
            binding modes to form eIF4E complexes that display differential 
            sensitivity to 4E-BP regulation.
  JOURNAL   Genes Dev. 2015 Sep 1;29(17):1835-49. doi: 10.1101/gad.269068.115. 
            Epub 2015 Aug 20.
  PUBMED    26294658
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.269068.115"; journalName: "Genes Dev"; date: 
            "2015-09-1- 1"; volume: "29"; issue: "17"; pages: "1835-49"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmMextli
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..54
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(77..94)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(104..145)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gatcctcgat cgaggctgat cagcgagctc aggccttaga aggcacagtc gaggctgatc
       61 agcgggttta aactcaatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga
      121 ggagagggtt agggataggc ttaccttcga accgcgggcc cttattcgtc cagagtctcg
      181 acctcatcgg cagccacaat gtctcgcttg gcggccgtct taatgctggc cagatactta
      241 tcaccatcga agcgctggct ctcatcctgc agatccttgt tgcgcagaat cgagggcgct
      301 gtctcctgca tcttttgcca gtcggtgggc agggtccacg agtgcggact catggagtag
      361 tagagcaagt gttcaatatc gtaagaaacg cgagatgcat tgcttttcaa ggaattggtg
      421 cctgccaaat cgtccaagtc acccttttcc cgagcgccct tggtctccgg cgtggactcc
      481 ttgcgcgaaa agtggctgcg acgcgatctg gccagtccat tctgattgct agtcgatgat
      541 cctccattgc tgggctcagc gaacacctcg tcgtcgcctt caccattgtt gttcgctgac
      601 gaggaaacaa cgcaattcag tccaatgcca ctgtccgtgc cgggcagcca aaattgcggc
      661 ggcccggctc cgggaagcgg agtcgatggc gtggtcacca gcgaagtgcc gtcgaaggcg
      721 gcgccagcct caatggatgc cagaaactcc gagctgctaa aatagtcatc gagcacgagt
      781 ttggccacgc gcactagttc gcagcagtcg ccggtgatct tgatcaggtg ctggcccacg
      841 ttgaccgtga acttgtattc acctacggaa gcatcgtttg tggaatatga gtgcattagc
      901 agctgttgat tggggcgcaa gactttaccg gcagcggctg tcgcctgagc atgtgccgcc
      961 gctgctgctg cggcggctac tgcggcgac
//
