LOCUS       sequence-152985-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pado with mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Kang BE, Baker BJ
  TITLE     Pado, a fluorescent protein with proton channel activity can 
            optically monitor membrane potential, intracellular pH, and map gap 
            junctions.
  JOURNAL   Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865.
  PUBMED    27040905
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-4"; volume: "6:23865. doi"; pages: " 
            10.1038/srep23865"
COMMENT     Sequence Label: Pado Red pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgc caccatggag ggattcgacg
      121 gttcagattt tagtcctcca gctgatttag ttggcgttga cggtgcagtc atgcggaacg
      181 tcgttgacgt cacgataaat ggtgacgtca ctgctccgcc gaaagctgcg ccaagaaaaa
      241 gtgaatcggt aaagaaagtt cattggaatg atgtagacca aggaccgagt gaaaaaccag
      301 agacaagaca ggaggaacga atagatatac ccgagatttc aggtctatgg tggggcgaga
      361 atgaacatgg agtggacgat gggagaatgg agatacctac tactggtgta ggtcgcgtcc
      421 agtttcggat ccgagccgtg attgatcatc tagggatgcg agtgttccac ctgaccatcg
      481 tgatcctgtg ctgcctggac ggcctgctgg tgatctgcgt gctgctgctg gagatcgagg
      541 ccctgaagct gaagagcagc agcgagctga ggcctaagct ggtgaacgtg cagttcgcca
      601 tcgagtgctg cagcctggcc atcgtgttcc tgttcgtggt ggagatccct ttcaagctgt
      661 ggatcttcgg ctgcaggatg ttcttccaca actggctgga gatcatcgac gccctggtgt
      721 gcctggtgag cttcgccgcc gacagctaca gcatcatcca ccacaccctg caccactaca
      781 ccaacaccga caggcctcag agccacatgc agaccagcca gagcaacgcc accaggggcc
      841 tgaccgagca cttccctgtg gaggagagcg ccgccagcaa caccatcgtg gacgccgccg
      901 ccctgctgat cctgttcagg ctgtggaggg tggtgaggat cttttattcc caccaacaaa
      961 tgaaggcttc aagcagaaga
//
