LOCUS       sequence-152987-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untagged EZH2-TALEN_Right to target the N-terminus of human EZH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: EZH2-TALEN_Right_No_FLAG
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..58)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(74..661)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt
       61 cgaagggccc ttaaaagttt atctcgccgt tattaaattt ccgtctgact tcctctaagg
      121 ttaatgtgcc ggctttaatc atttctccac caattaaaag ctcttctaca ctaagaacag
      181 ctccattaca attagtgata tgatttaatc gtgtaagctg agctttgtag tttcctttaa
      241 agtgaccact cacaaataaa aacttaaatt ccgttacaga agatggatag actttccacc
      301 attcattagg gttgatatgt ttgtttcgtg tttgattttc ttcgacatat cgttgcattt
      361 catctgcttg gccaattggc agattataac ctccgctata agctttagta tccacgatca
      421 caccgtaatc aataggagat ccgacagtat aaattgctcc gtccggtttc cttgatccac
      481 ccaaatgttt acctctatat ccataaactt tcataaaaaa ttccattacc ttcatttcaa
      541 gaattctatc ctgagtggaa tttctggcaa tttcaattaa ttcaatatat tcatgaggca
      601 catatttcaa tttatgacga agttcagatt tcttctcctc cagttcactt ttgactagtt
      661 gggatcccgc gactcgatgg gaagttctct cgggaatccg ccggttggtt cttttgatca
      721 atgcgggagc atgaggcaga ccctttttga ctgcatcgag cgcgggtcgt ccaccaagac
      781 atgccaacgc caccagatgg tcattcgtta acgcagccaa cgcgggatcg ggcctggaca
      841 attgggctac gatggactcc aaggctggcc gtcctccccc attagaagca atggcgacca
      901 cctgttcggg tgtcagtcca tgatcctggc acagtacagg cagcaggcgc tgtactgttt
      961 ccagcgcctg ctta
//
