LOCUS       sequence-152990-         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmThor-V5His6
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             427..468
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             478..495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(518..535)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    568..702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(592..611)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     646..665
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggcatggacg agctgtacaa gccaagcttg aattctgcag atatcatgtc cgcttcaccc
       61 accgcccgtc aagccatcac ccaggccctg cccatgatca ccaggaaggt tgtcatctcg
      121 gatccgatcc agatgcccga ggtgtactcc tcgacgcccg gcggaaccct ctactccacc
      181 actcctggag gcaccaaact tatctacgag cgggctttca tgaagaatct ccgtggctcc
      241 ccattgagcc aaactccgcc gtccaacgtg cccagttgct tgctgagggg cactccgcgt
      301 actcccttcc gcaagtgcgt gcccgtcccc acggaactga tcaagcagac caagtcgctg
      361 aagatcgagg accaggaaca gttccaactg gatctgcgct cgagtctaga gggcccgcgg
      421 ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
      481 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc
      541 tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt
      601 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      661 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      721 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      781 tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc
      841 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
      901 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tc
//
