LOCUS       sequence-152991-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Proton Channel Fluorescent Voltage Indicator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Kang BE, Baker BJ
  TITLE     Pado, a fluorescent protein with proton channel activity can 
            optically monitor membrane potential, intracellular pH, and map gap 
            junctions.
  JOURNAL   Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865.
  PUBMED    27040905
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-4"; volume: "6:23865. doi"; pages: " 
            10.1038/srep23865"
COMMENT     Sequence Label: Pado pcDNA3.1
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..739)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLE
                     FVTADGITHGMDELYK"
     primer_bind     complement(66..86)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     681..709
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgggccctct agactcgagt catcatttgt atagttcatc catgccatgt gtaatcccat
       61 cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa
      121 gagtagaagt tgtaaacagg taatggttgt ctggtaaaag gacgggccca tcgccaattg
      181 gagtattttg ttgataatgg tctgctagtt gaacgcctcc atcttcaatg ttgtgtctaa
      241 ttttgaagtt agctttgatt ccattctttt gtttgtctgc catgatgtac acctggtgat
      301 cgttatagtt gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac
      361 cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct
      421 tgtagttccc gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg
      481 cactcttgaa aaagtcatgc cgtttcatat gatctgggta tcttgaaaag cattgaacac
      541 cataagttaa agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa
      601 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa
      661 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa
      721 gttcttctcc tttactcatg cgatatcttc ttttgttctg tgatattgtt cttctgcttg
      781 aagccttcat ttgttggtgg gaataaaaga tcctcaccac cctccacagc ctgaacagga
      841 tcagcagggc ggcggcgtcc acgatggtgt tgctggcggc gctctcctcc acagggaagt
      901 gctcggtcag gcccctggtg gcgttgctct ggctggtctg catgtggctc tgaggcctgt
      961 cggtgttggt gtagtggtgc agggtgtggt gga
//
